Applications of Loop-Mediated Isothermal DNA Amplification
Applications of Loop-Mediated Isothermal DNA Amplification
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DOI：
10.1007/s12010-010-9088-8
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发表时间：
2011-04-01
影响因子：
3
通讯作者：
Shen, Zhiqiang
中科院分区：
文献类型：
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作者：
Fu, Shijun;Qu, Guanggang;Shen, Zhiqiang
近10年来，随着环介导等温扩增（loop-mediated isothermal amplification，LAMP）方法的发展，该方法以其简便、快速、高效、特异性强等优点在核酸分析中得到了广泛的应用。该方法采用DNA聚合酶和一组四种专门设计的引物，其识别靶DNA上总共六种不同的序列。不需要昂贵的设备来获得高水平的精确度，并且与常规PCR和实时PCR测定相比，制备步骤更少。本文简要综述了LAMP方法在病原微生物、转基因成分、肿瘤检测、胚胎性别鉴定等方面的应用。
During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation steps compared to conventional PCR and real-time PCR assays. This paper briefly summarized the applications of LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification.