Bacteriophytochromes are photochromic histidine kinases using a biliverdin chromophore
Bacteriophytochromes are photochromic histidine kinases using a biliverdin chromophore
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DOI：
10.1038/414776a
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发表时间：
2001-12-13
期刊：
影响因子：
64.8
通讯作者：
Vierstra, RD
中科院分区：
文献类型：
--
作者：
Bhoo, SH;Davis, SJ;Vierstra, RD
光敏色素包括植物中红光/远红光传感器的主要家族（1）。尽管光敏色素最初被认为仅限于光合生物（2，3），但我们最近在两种异养真细菌（耐辐射球菌和铜绿假单胞菌）中检测到光敏色素样蛋白（4）。在这里，我们表明，这些形式的一个广泛的家庭的细菌光敏色素（BphPs）的同源性，两个组件的传感器组氨酸激酶的一部分。植物光敏色素使用光敏色素作为发色团，而BphPs与胆绿素组装，胆绿素是血红素的直接分解产物，以产生由红光和远红光调制的光致变色激酶。在某些情况下，负责胆绿素合成的独特血红素加氧酶是BphP操纵子的一部分。该加氧酶与BphP脱辅基蛋白和血红素源的共表达足以在体内组装全BphP。它们在许多不同的细菌中的存在以及它们与胆绿素的简化组装都表明BphPs是光敏色素型光感受器的祖细胞。
Phytochromes comprise a principal family of red/far-red light sensors in plants(1). Although phytochromes were thought originally to be confined to photosynthetic organisms(2,3), we have recently detected phytochrome-like proteins in two heterotrophic eubacteria, Deinococcus radiodurans and Pseudomonas aeruginosa(4). Here we show that these form part of a widespread family of bacteriophytochromes (BphPs) with homology to two-component sensor histidine kinases. Whereas plant phytochromes use phytochromobilin as the chromophore, BphPs assemble with biliverdin, an immediate breakdown product of haem, to generate photochromic kinases that are modulated by red and far-red light. In some cases, a unique haem oxygenase responsible for the synthesis of biliverdin is part of the BphP operon. Co-expression of this oxygenase with a BphP apoprotein and a haem source is sufficient to assemble holo-BphP in vivo. Both their presence in many diverse bacteria and their simplified assembly with biliverdin suggest that BphPs are the progenitors of phytochrome-type photoreceptors.