Cross-linking of dermal sheep collagen using a water-soluble carbodiimide
Cross-linking of dermal sheep collagen using a water-soluble carbodiimide
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DOI：
10.1016/0142-9612(96)81413-x
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发表时间：
1996-04-01
期刊：
影响因子：
14
通讯作者：
Feijen, J
中科院分区：
文献类型：
--
作者：
Damink, LHHO;Dijkstra, PJ;Feijen, J
使用水溶性碳二亚胺1 - 乙基 - 3 -（3 - 二甲基氨丙基）碳二亚胺盐酸盐（EDC）开发了一种基于胶原蛋白的生物材料的交联方法。使用EDC交联涉及羧基的活化以产生O - 酰基异脲基团，其在与游离胺基反应后形成交联。用EDC处理绵羊真皮胶原蛋白（DSC）（E - DSC）得到的材料收缩温度（T - s）升高，游离胺基含量降低，表明发生了交联。在含EDC的交联溶液中添加N - 羟基琥珀酰亚胺（E/N - DSC）提高了交联速率。交联使非交联的DSC样品的T - s从56℃分别提高到E - DSC的73℃和E/N - DSC样品的86℃。对于这两种交联方法，都观察到游离胺基含量的降低与T - s的升高之间呈线性关系。E/N - DSC样品的拉伸强度和高应变模量在交联后分别从18 MPa降低到15 MPa和从26 MPa降低到16 MPa。E/N - DSC的断裂伸长率在交联后从142%提高到180%。
A cross-linking method for collagen-based biomaterials was developed using the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC). Cross-linking using EDC involves the activation of carboxylic acid groups to give O-acylisourea groups, which form cross-links after reaction with free amine groups. Treatment of dermal sheep collagen (DSC) with EDC (E-DSC) resulted in materials with an increased shrinkage temperature (T-s) and a decreased free amine group content, showing that cross-linking occurred. Addition of N-hydroxysuccinimide to the EDC-containing cross-linking solution (E/N-DSC) increased the rate of cross-linking. Cross-linking increased the T-s of non-cross-linked DSC samples from 56 to 73 degrees C for E-DSC and to 86 degrees C for E/N-DSC samples, respectively. For both cross-linking methods a linear relation between the decrease in free amine group content and the increase in T-s was observed. The tensile strength and the high strain modulus of E/N-DSC samples decreased upon cross-linking from 18 to 15 MPa and from 26 to 16 MPa, respectively. The elongation at break of E/N-DSC increased upon cross-linking from 142 to 180%.