The development of a recombinant Babesia vaccine.
The development of a recombinant Babesia vaccine.
复制标题
重组巴贝虫疫苗的开发。
DOI：
10.1016/0304-4017(92)90138-y
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发表时间：
1992
影响因子：
2.6
通讯作者：
M. White
中科院分区：
文献类型：
--
作者：
I. G. Wright;R. Casu;M. Commins;B. Dalrymple;K. Gale;B. Goodger;P. Riddles;D. Waltisbuhl;I. Abetz;D. Berrie;Y. Bowles;C. Dimmock;T. Hayes;H. Kalnins;G. Leatch;R. McCrae;P. E. Montague;I. Nisbet;F. Parrodi;J. Peters;P. Scheiwe;W. J. Smith;K. Rode;M. White
研究表明，牛巴贝斯虫的粗提取物对易感牛产生的保护作用相当于自然感染产生的保护作用。对粗料进行了系统分离，并在成牛中进行了多次顺序接种/攻毒实验。然后用单克隆抗体亲和层析纯化保护组分中的抗原。因此，确定了三种高度保护性（减少寄生虫病95%以上）抗原。这些抗原都不是免疫优势抗原；许多免疫优势抗原被鉴定出来，所有抗原都是免疫抑制和/或无保护性的。克隆了3种保护性抗原，分别以β-半乳糖苷酶和谷胱甘肽- s -转移酶（GST）融合蛋白表达。其中两种疫苗GST-12D3和GST-11C5联合使用时，其保护作用几乎与之前市售减毒活疫苗一样。第三种抗原（21B4）的短片段也被证明具有保护作用。在两种抗原中，重复片段已被证明是非保护性的，而第三种抗原（12D3）不包含重复结构域。这些抗原的同源物存在于其他巴贝虫物种中，预计这些抗原可能是针对这些物种的保护性疫苗的候选抗原。
Crude extracts ofBabesia bovisparasites were shown to induce levels of protection in susceptible cattle equivalent to that resulting from natural infection. The crude material was systematically fractionated and tested in numerous sequential vaccination/challenge experiments in adult cattle. Antigens in protective fractions were then purified by affinity chromatography with monoclonal antibodies. Three highly protective (more than 95% reduction in parasitaemias) antigens were thus identified. None of these antigens was immunodominant; a number of immunodominant antigens were identified and all were immunosuppressive and/or non-protective.The three protective antigens were cloned and expressed as either β-galactosidase or glutathione-S-transferase (GST) fusion proteins. Two of these, GST-12D3 and GST-11C5, when used in combination were almost as protective as has been previously shown for the commercially available live attenuated vaccine. A short fragment of a third antigen (21B4) has also been shown to be protective. In two of the antigens, repetitive segments have been shown to be non-protective while the third antigen (12D3) does not contain repetitive domains.Homologues of these antigens exist in otherBabesiaspecies and it is anticipated that these may be candidate antigens for protective vaccines against those species.