Intestinal microbial profiles in extremely preterm infants with and without necrotizing enterocolitis
Intestinal microbial profiles in extremely preterm infants with and without necrotizing enterocolitis
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DOI：
10.1111/apa.12059
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发表时间：
2013-02-01
期刊：
影响因子：
3.8
通讯作者：
Lilja, Helene Engstrand
中科院分区：
文献类型：
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作者：
Normann, Erik;Fahlen, Annika;Lilja, Helene Engstrand
目的坏死性小肠结肠炎(NEC)是早产儿最严重的并发症之一。肠道微生物区系在NEC的发展中所起的作用需要阐明。方法应用条形码焦磷酸测序技术对极早产儿粪便标本进行前瞻性单中心病例对照研究。在对样本进行分析之前，10名患者被诊断为NEC，并在性别、胎龄和分娩方式方面与健康对照组匹配。结果菌群以肠球菌、杆菌属、肠杆菌科为主。尽管没有统计学意义，但在NEC患者的早期时间点检测到了较高的杆菌和肠杆菌科相对丰度，而健康对照组的微生物区系更多地是以肠球菌为主。NEC患者与对照组肠道微生物区系多样性较低，差异无统计学意义。在16名健康对照组中，在出生后的头几周，粪便菌群以肠球菌和芽孢杆菌属为主，然后由肠杆菌科取代。结论NEC患者的肠道菌群组成没有明显差异，但仍有一些发现需要在后续研究中进一步研究。
Aim Necrotizing enterocolitis (NEC) represents one of the gravest complications in premature infants. The suggested role of intestinal microbiota in the development of NEC needs to be elucidated. Methods This prospective single-centre casecontrol study applied barcoded pyrosequencing to map the bacterial composition of faecal samples from extremely preterm infants. Ten patients were diagnosed with NEC and matched to healthy controls with regard to sex, gestational age and mode of delivery prior to analysis of the samples. Results Enterococcus, Bacillales and Enterobacteriaceae dominated the flora. Although not statistically significant, a high relative abundance of Bacillales and Enterobacteriaceae was detected at early time points in patients developing NEC, while healthy controls had a microbiota more dominated by Enterococcus. A low diversity of intestinal microbial flora was found without any differences between NEC patients and controls. In 16 healthy controls, Firmicutes (Enterococcus and Bacillales) dominated the faecal flora during the first weeks after birth and were then succeeded by Enterobacteriaceae. Conclusion No significant differences in the composition of intestinal microbiota of patients developing NEC were detected; however, some findings need to be scrutinized in subsequent studies.