Anteroventrally localized activity in the optic vesicle plays a crucial role in the optic development
Anteroventrally localized activity in the optic vesicle plays a crucial role in the optic development
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DOI：
10.1016/j.ydbio.2008.03.010
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发表时间：
2008-05-15
影响因子：
2.7
通讯作者：
Araki, Masasuke
中科院分区：
文献类型：
--
作者：
Hirashima, Miki;Kobayashi, Takuma;Araki, Masasuke
脊椎动物的眼睛是由视泡（OV）通过与周围组织的协调相互作用而发育而来的，视泡是前脑的一种横向扩张结构。然后OV内陷形成视杯，同时透镜基板发育为透镜囊泡。这些方面在早期阶段的特点脊椎动物眼睛的形成和控制适当的背腹协调。在本研究中，我们进行了手术操作，在小鸡OV去除背侧或腹侧的一半，并检查了剩余的OV的发展。结果表明，背侧和腹侧的OV有一个明显不同的发展模式。当背侧的一半被删除，剩下的腹侧的OV发展成一个完整的眼睛，而背侧的OV发展成一个色素囊泡组成的视网膜色素上皮单独。这些结果表明，OV的腹侧部分保留了发育整个眼睛结构的潜力，并在适当的眼睛发育中起着至关重要的作用。在随后对早期鸡胚的操作中，发现只有OV的前腹象限具有发育整个眼睛的潜力，并且OV的其他部分没有类似的活性。Fgf8的表达定位在这一部分，没有观察到Fgf8的表达，当腹侧OV被删除的OV。这些结果表明，前腹侧部分的OV起着至关重要的作用，在眼睛的正常发展，可能产生的背腹梯度的信号蛋白的眼原基内。(C)2008年爱思唯尔公司All rights reserved.
The vertebrate eye develops from the optic vesicle (OV), a laterally protrusive structure of the forebrain, by a coordinated interaction with surrounding tissues. The OV then invaginates to form an optic cup, and the lens placode develops to the lens vesicle at the same time. These aspects in the early stage characterize vertebrate eye formation and are controlled by appropriate dorsal-ventral coordination. In the present study, we performed surgical manipulation in the chick OV to remove either the dorsal or ventral half and examined the development of the remaining OV. The results show that the dorsal and ventral halves of the OV have a clearly different developmental pattern. When the dorsal half was removed, the remaining ventral OV developed into an entire eye, while the dorsal OV developed to a pigmented vesicle consisting of retinal pigmented epithelium alone. These results indicate that the ventral part of the OV retains the potency to develop the entire eye structure and plays an essential role in proper eye development. In subsequent manipulations of early chick embryos, it was found that only the anterior ventral quadrant of the OV has the potential to develop the entire eye and that no other part of the OV has a similar activity. Fgf8 expression was localized in this portion and no Fgf8 expression was observed within the OV when the ventral OV was removed. These results suggest that the anterior ventral portion of the OV plays a crucial role in the proper development of the eye, possibly generating the dorsal-ventral gradients of signal proteins within the eye primordium. (C) 2008 Elsevier Inc. All rights reserved.