Disease-Specific Expression of Host Genes During Downy Mildew Infection of Arabidopsis
Disease-Specific Expression of Host Genes During Downy Mildew Infection of Arabidopsis
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DOI：
10.1094/mpmi-22-9-1104
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发表时间：
2009-09-01
影响因子：
3.5
通讯作者：
Van den Ackerveken, Guido
中科院分区：
文献类型：
--
作者：
Huibers, Robin P.;de Jong, Mark;Van den Ackerveken, Guido
在这里，我们报告的拟南芥基因的识别过程中，诱导兼容，但不兼容的相互作用与霜霉病病原体透明霜霉病菌arabidopsidis。这组所谓的相容性特异性（CS）基因与在相容性和不相容性相互作用期间差异表达的一大组防御相关基因形成对比。在17个已鉴定的CS基因中，有6个属于乙烯反应因子（ERF）家族的转录因子基因，表明这些ERF基因在植物的亲和性过程中发挥作用。大多数CS基因在响应各种形式的非生物胁迫时受到差异调节。CS基因的计算机模拟分析揭示了在其启动子区域中脱水反应元件/C重复序列结合因子（DREB 1A/CBF 3）结合位点和EveningElement基序的过度表达。CS-ERF与CBF转录因子密切相关，可能与CS基因中的DREB 1A/CBF 3启动子元件结合。CS基因的转录水平在接种后2至3天达到峰值，此时病原体生长最高，并在感染后期下降。几个CS基因的诱导被发现是分离物特异性的。这表明鉴定的CS基因可能是霜霉病效应蛋白促进疾病易感性的直接或间接靶标。
Here, we report on the identification of Arabidopsis genes that are induced during compatible but not during incompatible interactions with the downy mildew pathogen Hyaloperonospora arabidopsidis. This set of so-called compatible specific (CS) genes contrasts with the large group of defense-associated genes that is differentially expressed during both compatible and incompatible interactions. From the 17 identified CS genes, 6 belong to the ethylene response factor (ERF) family of transcription factor genes, suggesting that these ERF have a role during compatibility. The majority of CS genes are differentially regulated in response to various forms of abiotic stress. In silico analysis of the CS genes revealed an over-representation of dehydration-responsive element/C-repeat binding factor (DREB1A/CBF3) binding sites and EveningElement motifs in their promoter regions. The CS-ERF are closely related to the CBF transcription factors and could potentially bind the DREB1A/CBF3 promoter elements in the CS genes. Transcript levels of CS genes peak at 2 to 3 days postinoculation, when pathogen growth is highest, and decline at later stages of infection. The induction of several CS genes was found to be isolate specific. This suggests that the identified CS genes could be the direct or indirect targets of downy mildew effector proteins that promote disease susceptibility.