Classification of prostaglandin receptors based on coupling to signal transduction systems.
Classification of prostaglandin receptors based on coupling to signal transduction systems.
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基于与信号转导系统耦合的前列腺素受体分类。
DOI：
10.1042/bj2630769
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Yamaguchi,DT
中科院分区：
文献类型：
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作者：
Muallem,S;Merritt,BS;Green,J;Kleeman,CR;Yamaguchi,DT
在成骨性骨肉瘤细胞系UMR-106-01中，广谱的洋地黄素（PG）刺激环AMP的产生和游离胞质Ca 2+浓度[（Ca 2 +]i）的增加，其具有与成骨细胞相容的特性。利用PG刺激的第二信使cAMP和[Ca ~（2+）]i的测定，提出了一种PG受体分类的方法。UMR-106-01细胞显示PG受体的三个亚类。一种受体与PGF 2 α、PGD 2和血栓烷B2（TxB 2）相互作用，以增加[Ca 2 +]i。第二个受体与PGE 2、PGE 1、PGI 2、PGA 2和6-氧代-PGF 1 α结合，通过刺激第二个单独的磷脂酶C库来增加[Ca 2 +]i。第三种受体接受PGE 2、PGE 1、PGA 2、PGI 2，并在较小程度上接受PGF 2 α、PGD 2和TxB 2，以增加环AMP。这样的分类系统可以适用于通过诱导细胞第二信使的变化而响应多种PG的其他细胞。
A wide spectrum of prostaglandins (PG) stimulate both the production of cyclic AMP and an increase in free cytosolic Ca2+ concentration [(Ca2+]i) in the osteogenic osteosarcoma cell line, UMR-106-01, which has characteristics compatible with osteoblasts. Using PG-stimulated determinations of the second messengers cyclic AMP and [Ca2+]i, a method for classification of PG receptors is presented. UMR-106-01 cells demonstrate three subclasses of PG receptors. One receptor interacts with PGF2 alpha, PGD2, and thromboxane B2 (TxB2) to increase [Ca2+]i. A second receptor binds PGE2, PGE1, PGI2, PGA2 and 6-oxo-PGF1 alpha to increase [Ca2+]i by stimulation of a second separate phospholipase C pool. A third receptor accepts PGE2, PGE1, PGA2, PGI2 and to a lesser extent PGF2 alpha, PGD2 and TxB2 to increase cyclic AMP. Such a classification system may be applicable to other cells responding to multiple PGs by inducing changes in cellular second messengers.