Modification of U6 spliceosomal RNA is guided by other small RNAs
Modification of U6 spliceosomal RNA is guided by other small RNAs
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DOI：
10.1016/s1097-2765(00)80161-6
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发表时间：
1998-11-01
期刊：
影响因子：
16
通讯作者：
Steitz, JA
中科院分区：
文献类型：
--
作者：
Tycowski, KT;You, ZH;Steitz, JA
脊椎动物剪接体snRNA通过假尿苷化和2 ′-O-甲基化高度修饰。我们已经鉴定了新的保守的小RNA，其可以指导在U6 snRNA的A47和C77处添加两个甲基。这些指导RNA，mgU 6 -47（U6 snRNA残基47的甲基化指导）和mgU 6 -77含有盒C、C '、D和D'，并与原纤蛋白缔合。每个RNA可以与U6 snRNA形成双链体，定位A47和077用于2 '-O-甲基化。mgU 6 -77的反义元件也可以定位28 S rRNA的C2970进行2 '-O-甲基化。从非洲爪蟾卵母细胞中消耗mgU 6 -77阻止了U6中C77和28 S中C2970的2 '-O-甲基化;甲基化可以通过注射体外转录的mgU 6 -77来恢复。因此，mgU 6 -77似乎在两种不同类型的细胞RNA（snRNA和rRNA）的2 '-O-甲基化中起作用。
The vertebrate spliceosomal snRNAs are highly modified by pseudouridylation and 2'-O-methylation, We have identified novel conserved small RNAs that can direct addition of two methyl groups in U6 snRNA, at A47 and C77. These guide RNAs, mgU6-47 (methylation guide for U6 snRNA residue 47) and mgU6-77 contain boxes C, C', D, and D' and associate with fibrillarin. Each RNA can form a duplex with U6 snRNA positioning A47 and 077 for 2'-O-methylation. The antisense element of mgU6-77 can also position C2970 of 28S rRNA for 2'-O-methylation. Depletion of mgU6-77 from Xenopus oocytes prevents 2'-O-methylation of both C77 in U6 and C2970 in 28S; methylation can be restored by injecting in vitro transcribed mgU6-77. Thus, mgU6-77 appears to function in the 2'-O-methylation of two distinct classes of cellular RNA, snRNA,and rRNA.