Detection of Fel d 1-immunoglobulin G immune complexes in cord blood and sera from allergic and non-allergic mothers
Detection of Fel d 1-immunoglobulin G immune complexes in cord blood and sera from allergic and non-allergic mothers
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DOI：
10.1034/j.1399-3038.2001.012002059.x
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发表时间：
2001-04-01
影响因子：
4.4
通讯作者：
Björkstén, B
中科院分区：
文献类型：
--
作者：
Casas, R;Björkstén, B
众所周知，大多数脐带血样本中都存在胎儿来源的 T 细胞对吸入性过敏原的反应。这些免疫反应可以通过肽的跨胎盘通道来解释，无论是作为游离抗原还是与免疫球蛋白 G (IgG) 的复合物，为胎儿提供启动子宫内已存在的 T 细胞系统的触发器。本研究的目的是调查脐带血和母体血清中主要猫过敏原 Fel d I 与 IgG 复合物的存在情况。通过使用放大酶联免疫吸附测定 (ELISA)，研究了 75 名母亲（38 名过敏，37 名非过敏）的血清样本及其婴儿的脐带血中是否存在 Fel d 1-IgC 免疫复合物 (IC)。使用三种针对 Fel d 1 的单克隆抗体进行包被。通过抑制实验证实了该方法的特异性。在 45% 过敏母亲和 49% 非过敏母亲的血清中检测到 Fel d 1-IgG 的 IC，在她们的婴儿中分别检测到 34% 和 41%。因此，IC 的患病率和水平均不受母亲过敏的影响。低水平的经胎盘转移的 IC 可以为胎儿提供启动 T 细胞对吸入性过敏原反应的信号。然而，这与过敏性疾病没有必然关系。氏族。
It is an established fact that T-cell responses of fetal origin to inhalant allergens are present in most cord blood samples. These immune responses could be explained by trans-placental passage of peptides, either as free antigens or in complexes with immunoglobulin G (IgG), providing the fetus with a trigger for priming the T-cell system already present in utero. The aim of this study was to investigate the presence of the major cat allergen, Fel d I, in complexes with IgG in cord blood and maternal sera. Serum samples from 75 mothers (38 allergic, 37 nonallergic), and cord blood from their infants, were investigated for the presence of Fel d 1-IgC immune complexes (ICs) by using an amplified enzyme-linked immunosorbent assay (ELISA). Three monoclonal antibodies to Fel d 1 were used for coating. The specificity of the method was confirmed by inhibition experiments. ICs of Fel d 1-IgG were detected in the sera of 45% allergic and 49% non-allergic mothers, and in, respectively, 34% and 41% of their infants. Therefore, neither the prevalence nor the level of ICs were affected by maternal allergy. Low levels of trans-placentally transferred ICs can provide the fetus with a signal for the priming of T-cell responses to inhalant allergens. However, this is not necessarily related to allergic disease. gens.