Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4.
Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4.
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DOI：
10.1038/ncomms4080
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发表时间：
2014
影响因子：
16.6
通讯作者：
Waldor MK
中科院分区：
文献类型：
--
作者：
Munera D;Ritchie JM;Hatzios SK;Bronson R;Fang G;Schadt EE;Davis BM;Waldor MK
The outbreak of diarrhea and hemolytic uremic syndrome that occurred in Germany in 2011 was caused by a Shiga toxin-producing enteroaggregative Escherichia coli (EAEC) strain. The strain was classified as EAEC due to the presence of a plasmid (pAA) that mediates a characteristic pattern of aggregative adherence on cultured cells, the defining feature of EAEC that has classically been associated with virulence. Here, we describe an infant rabbit-based model of intestinal colonization and diarrhea caused by the outbreak strain, which we use to decipher the factors that mediate the pathogen’s virulence. Shiga toxin is the key factor required for diarrhea. Unexpectedly, we observe that pAA is dispensable for intestinal colonization and development of intestinal pathology. Instead, chromosome-encoded autotransporters are critical for robust colonization and diarrheal disease in this model. Our findings suggest that conventional wisdom linking aggregative adherence to EAEC intestinal colonization is false for at least a subset of strains.
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影响因子：
6.7
作者：
Ritchie JM;Rui H;Zhou X;Iida T;Kodoma T;Ito S;Davis BM;Bronson RT;Waldor MK
通讯作者：
Waldor MK
DOI：
10.1056/nejmoa1106920
发表时间：
2011-08-25
期刊：
The New England journal of medicine
影响因子：
--
作者：
Rasko DA;Webster DR;Sahl JW;Bashir A;Boisen N;Scheutz F;Paxinos EE;Sebra R;Chin CS;Iliopoulos D;Klammer A;Peluso P;Lee L;Kislyuk AO;Bullard J;Kasarskis A;Wang S;Eid J;Rank D;Redman JC;Steyert SR;Frimodt-Møller J;Struve C;Petersen AM;Krogfelt KA;Nataro JP;Schadt EE;Waldor MK
通讯作者：
Waldor MK
DOI：
10.1073/pnas.1219076110
发表时间：
2013-03-05
影响因子：
11.1
作者：
Pavlova, Olga;Peterson, Janine H.;Bernstein, Harris D.
通讯作者：
Bernstein, Harris D.
影响因子：
3.1
作者：
DONNENBERG, MS;KAPER, JB
通讯作者：
KAPER, JB
影响因子：
4.2
作者：
Dautin N
通讯作者：
Dautin N