Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8)
Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8)
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DOI：
10.1006/bbrc.1998.8895
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发表时间：
1998-06-29
影响因子：
3.1
通讯作者：
Takigawa, M
中科院分区：
文献类型：
--
作者：
Nishida, T;Nakanishi, T;Takigawa, M
结缔组织生长因子（CTGF）的特异性受体的存在下，证明在人软骨肉瘤衍生的软骨细胞系，HCS-2/8。未标记的CTGF可抑制I-125标记的重组CTGF与HCS-2/8细胞的结合，而PDGF-BB和bFGF则不抑制I-125标记的重组CTGF与HCS-2/8细胞的结合。Scatchard分析揭示了细胞上存在两类结合位点，Rd值为18.6和259 nM。I-125-CTGF-受体复合物在加入未标记的CTGF后几乎完全消失，而在加入PDGF-BB或bFGF后则不消失。此外，I-125-CTGF-受体复合物与抗CTGF抗血清发生免疫沉淀，而与抗PDGF受体抗血清不发生免疫沉淀。这些发现表明CTGF直接与HCS-2/8细胞上的特异性受体分子结合。(C)北京：科学出版社.
The presence of receptors specific for connective tissue growth factor (CTGF) was demonstrated on a human chondrosarcoma-derived chondrocytic cell Line, HCS-2/8. The binding of I-125-labeIed recombinant CTGF to HCS-2/8 cells was inhibited by unlabeled CTGF but not by PDGF-BB or bFGF. Scatchard analysis revealed the presence of two classes of binding sites with Rd values of 18.6 and 259 nM on cells. A cross-linking study revealed the formation of I-12S-CTGF-receptor complex with an apparent molecular weight of 280 kDa, The I-125-CTGF-receptor complex disappeared almost completely on the addition of unlabeled CTGF but not PDGF-BB or bFGF, In addition, the I-125-CTGF-receptor complex was immunoprecipitated with anti-CTGF antiserum but not with anti-PDGF receptor antiserum. These findings suggest that CTGF directly binds to specific receptor molecules on HCS-2/8 cells. (C) 1998 Academic Press.