SHORT-TERM AND LONG-TERM EVALUATION OF NORMAL AND ABNORMAL HUMAN THYROID-CELLS IN MONOLAYER-CULTURE
SHORT-TERM AND LONG-TERM EVALUATION OF NORMAL AND ABNORMAL HUMAN THYROID-CELLS IN MONOLAYER-CULTURE
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DOI：
10.1111/j.1365-2265.1985.tb01106.x
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
FRIEDMAN, EW
中科院分区：
文献类型：
--
作者：
DAVIES, TF;PLATZER, M;FRIEDMAN, EW
我们研究了正常和异常的人甲状腺细胞的TSH反应性，在短期内培养高血清浓度和长达6个月的低血清，化学定义，培养基。来自正常人甲状腺组织（n = 9）、多结节性甲状腺肿（n = 6）、良性滤泡性腺瘤（n = 6）和分化的甲状腺癌（n = 3）的细胞形成汇合的单层，当在培养7天通过环AMP的细胞内反应评估时，所述细胞对浓度大于25 μ U/ml的牛TSH（bTSH）敏感。这种敏感性低于用来源于Fisher大鼠甲状腺的持续增殖甲状腺细胞系（FRTL-5）观察到的敏感性，该细胞系对低至5-10 μ U/ml的bTSH浓度有反应。来自碘/抗甲状腺药物处理的Graves甲状腺组织的人细胞（n = 6）比正常细胞更不敏感，需要高达500 μ U/ml bTSH来增加细胞内环AMP，并且低分化甲状腺癌细胞（n = 3）对bTSH没有反应。在用于FRTL-5细胞的化学成分确定的培养基中，正常和滤泡性腺瘤细胞的长期人甲状腺培养物不存在成纤维细胞生长，并且继续以单层形式存在，没有显著的滤泡形成。这些细胞在连续培养4、13和26周后测试时对bTSH刺激保持高度敏感。在有利于大鼠甲状腺细胞快速生长的条件下，所有这些细胞制剂都不能增殖。这些数据表明，虽然文献中描述的甲状腺细胞培养条件不允许人甲状腺细胞增殖，但它们确实允许在体外评估其功能状态，这可能导致对甲状腺细胞病理生理学的进一步理解。
We have investigated the TSH responsiveness of normal and abnormal human thyroid cells cultured in the short term with high serum concentrations and for up to 6 months in a low serum, chemically-defined, medium. Cells from normal human thyroid tissue (n = 9), multinodular goitre (n = 6), benign follicular adenomata (n = 6), and differentiated thyroid carcinoma (n = 3) formed confluent monolayers which were sensitive to bovine TSH (bTSH) in concentrations greater than 25 .mu.U/ml when assessed by the intracellular response of cyclic AMP at 7 d of culture. Such sensitivity was less than that observed with a continuously proliferating thyroid cell line (FRTL-5) derived from Fisher rat thyroid and which responded to concentrations of bTSH as low as 5-10 .mu.U/ml. Human cells derived from iodine/antithyroid drug treated Graves'' thyroid tissue (n = 6) were less sensitive than normal cells requiring up to 500 .mu.U/ml bTSH to increase intracellular cyclic AMP and poorly differentiated thyroid cancer cells (n = 3) failed to respond to bTSH. Long-term human thyroid cultures of normal and follicular adenoma cells in the chemically-defined medium used for the FRTL-5 cells had absent fibroblast growth and continued in monolayer form without significant follicle formation. These cells remained highly sensitive to bTSH stimulation when tested after 4, 13, and 26 weeks of continuous culture. All such cell preparations failed to proliferate under conditions which favored the rapid growth of the rat thyroid cells. These data demonstrated that while thyroid cell culture conditions described in the literature do not permit proliferation of human thyroid cells, they do allow an assessment of their functional state in vitro which may lead to a further understanding of thyroid cell pathophysiology.