Activation of adenylate cyclase by vanadate
Activation of adenylate cyclase by vanadate
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钒酸盐激活腺苷酸环化酶
作者：
U. Schwabe;C. Puchstein;H. Hannemann;E. Söchtig
VANADATE已被确定为（Na，K）ATPases1,2的有效抑制剂。在最佳条件（28 mM Mg2+）2下，40 nM正钒酸钠（Na2VO4）对狗肾（Na，K） atp酶的抑制作用达到50%。由于钒酸盐最初被认为是肌源性ATP制备s2以及马和兔骨骼肌中的抑制剂，它被认为是内源性（Na，K） ATPase2-4的调节剂。在低ATP浓度的腺苷酸环化酶研究中，我们尝试使用钒酸盐作为抑制ATP酶的工具。我们在这里报道，钒酸盐引起腺苷酸环化酶活性的显著刺激，从大鼠脂肪细胞分离的膜，并产生最大的激活，即使在缺乏激素。
VANADATE has been identified as a potent inhibitor of (Na,K)ATPases1,2. The (Na,K)ATPase of dog kidney is inhibited 50% by 40 nM sodium orthovanadate (Na2VO4) in optimal conditions (28 mM Mg2+)2. As vanadate was originally identified as an inhibitor in muscle-derived ATP preparations2 and in equine and rabbit skeletal muscles, it has been suggested as endogenous regulator of (Na,K)ATPase2–4. We have tried to use vanadate as a tool to inhibit ATPases in adenylate cyclase studies when working at low ATP concentrations. We report here that vanadate causes marked stimulation of adenylate cyclase activity in membranes isolated from rat fat cells and produces maximal activation even in the absence of hormones.