Improved immobilization of biomolecules to quinone-rich polydopamine for efficient surface functionalization
Improved immobilization of biomolecules to quinone-rich polydopamine for efficient surface functionalization
复制标题
改进生物分子在富含醌的聚多巴胺上的固定，以实现有效的表面功能化
DOI：
10.1016/j.colsurfb.2013.01.033
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发表时间：
2013-06-01
影响因子：
5.8
通讯作者：
Huang, Nan
中科院分区：
文献类型：
--
作者：
Luo, Rifang;Tang, Linlin;Huang, Nan
聚多巴胺（PDA）是一种具有生物活性的高分子材料，通过固定生物分子而具有广泛的应用前景。在这项工作中，开发了一种新的方法，使用PDA的改进的生物分子固定。将薄PDA层策略性地涂覆到316L不锈钢上并在150 ℃下热处理（PDA-Th150）。随后，氨基封端的聚乙二醇（mPEG-NH2）或血管内皮生长因子（VEGF）固定到PDA和PDA-Th150表面。衰减全反射-傅里叶变换-红外光谱（ATR-FTIR）和X射线光电子能谱（XPS）的结果表明，奎宁在PDA-Th150表面的覆盖率较高。血小板粘附实验和内皮细胞增殖实验表明，功能化PDA-Th150显著提高了其固定mPEG-NH2或VEGF的能力。这种新的方法也可用于在不同的金属器件上有效地固定生物分子。由爱思唯尔公司出版
Polydopamine (PDA), a bio-inspired polymer, has been very attractive for diverse functional applications by immobilizing biomolecules. In this work, a novel approach of using PDA for improved biomolecule immobilization was developed. A thin PDA layer was strategically coated onto 316L stainless steel and thermally treated at 150 degrees C (PDA-Th150). Subsequently, amino-terminated polyethylene glycol (mPEG-NH2) or vascular endothelial growth factor (VEGF) was immobilized onto PDA and PDA-Th150 surface. The results of attenuated total reflection-Fourier transform-infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) showed higher coverage of quinine on PDA-Th150 surface. The functionalized PDA-Th150 significantly improved its ability of immobilizing mPEG-NH2 or VEGF, as shown by platelet adhesion test and endothelial,cell proliferation experiments. This novel approach may also be used for efficient immobilization of biomolecules on different metal devices. Published by Elsevier B.V.