Protective effect of Ganoderma atrum polysaccharides in acute lung injury rats and its metabolomics
Protective effect of Ganoderma atrum polysaccharides in acute lung injury rats and its metabolomics
复制标题
灵芝多糖对急性肺损伤大鼠的保护作用及其代谢组学
DOI：
10.1016/j.ijbiomac.2019.10.010
复制
发表时间：
2020-01-01
影响因子：
8.2
通讯作者：
Xie, Ming-yong
中科院分区：
文献类型：
--
作者：
Li, Lu;Fu, Wang-wei;Xie, Ming-yong
本研究旨在探讨灵芝多糖（PSG）对急性肺损伤（ALI）大鼠的作用及其机制。结果表明，PSG通过维持肺组织，降低血清和肺组织中促炎细胞因子和NO的水平，对ALI具有保护作用。此外，本研究基于UPLC-Triple-TOF/MS代谢组学分析，进一步评估了PSG在大鼠体内的代谢作用。与对照组相比，ALI组血清代谢标志物为LysoPC（18:2）、LPA（18:1）、牛磺酸、l -组氨酸和l -色氨酸。此外，生物学途径分析表明，PSG处理显著改变了ALI大鼠的组氨酸代谢、氮代谢、色氨酸和部分甘油磷脂代谢。此外，摄入PSG后，ALI大鼠肠道微生物代谢物短链脂肪酸有所改善。总之，PSG可以控制ali诱导的异常炎症，其机制与抑制促炎介质的释放和逆转代谢途径紊乱有关。(C) 2019 Elsevier B.V.版权所有
The present study aimed to evaluate effect of Ganoderma atrum polysaccharide (PSG) on acute lung injury (ALI) rats and its mechanisms. Results showed that PSG exhibited protective effects against ALI by maintaining pulmonary histology, reducing levels of pro-inflammatory cytokines and NO both in serum and lung tissue. Moreover, this study further evaluated the metabolic effects of PSG based on UPLC-Triple-TOF/MS metabolomics analysis in rats. Compared with control group, LysoPC (18:2), LPA (18:1), taurocholic acid, L-histidine, and L-tryptophan were identified as metabolic biomarkers in serum of ALI group. Furthermore, biological pathways analysis demonstrated that histidine metabolism, nitrogen metabolism, tryptophan and part glycerophospholipids metabolism were notably modified by PSG treatment in ALI rats. Additionally, improved gut microbial metabolite short-chain fatty acids were found after intake of PSG in ALI rat. Altogether, PSG could control ALI-induced aberrant inflammation and its mechanisms were linked to inhibit release of pro-inflammatory mediators and reverse metabolic pathway disturbances. (C) 2019 Elsevier B.V. All rights reserved.