Enzymic properties of intestinal aminopeptidase P: A new continuous assay
Enzymic properties of intestinal aminopeptidase P: A new continuous assay
复制标题
肠氨肽酶 P 的酶学特性：一种新的连续测定
DOI：
10.1016/0014-5793(88)80891-3
复制
发表时间：
1988
期刊：
影响因子：
3.5
通讯作者：
H. Demuth
中科院分区：
文献类型：
--
作者：
J. Lasch;R. Koelsch;T. Steinmetzer;U. Neumann;H. Demuth
以底物Gly‐Pro‐Pro‐pNA和DPP IV为辅助酶，建立了连续光度法测定氨基肽酶P活性的方法。本实验旨在评价肠刷边氨基肽酶P的动力学参数和抑制谱。
A continuous photometric assay of aminopeptidase P activity was developed which is based on a coupled enzymic assay with the substrate Gly‐Pro‐Pro‐pNA and DPP IV as auxiliary enzyme. This assay was used to evaluate the kinetic parameters and inhibitory profile of intestinal brush border aminopeptidase P.