Adhesive and mechanical properties of hydrogels influence neurite extension
Adhesive and mechanical properties of hydrogels influence neurite extension
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DOI：
10.1002/jbm.a.30203
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发表时间：
2005-01-01
影响因子：
4.9
通讯作者：
Mann, BK
中科院分区：
文献类型：
--
作者：
Gunn, JW;Turner, SD;Mann, BK
研究了与生物活性配体缀合的光聚合聚乙二醇（PEG）水凝胶在周围神经再生应用中作为支架的用途。 PEG水凝胶的生物活性和机械性能可以分别通过生物活性因子（粘附配体、蛋白水解位点、生长因子）的整合和PEG浓度的改变来定制。对于周围神经再生，确定改善神经突延伸所需的生物活性分子的类型和浓度非常重要。在这项研究中，细胞粘附配体（RGDS、IKVAV 和 YIGSR）共价连接到 PEG 水凝胶上。所使用的细胞粘附配体的类型和浓度都会影响神经突的延伸。 PC12 细胞在掺有 RGDS 的水凝胶上的延伸比 IKVAV 更大，并且每种配体的最佳浓度不同。细胞粘附在带有 YIGSR 的水凝胶上，但不延伸神经突。细胞不粘附于含有 RGES 的水凝胶上。此外，这些配体的不同组合不同程度地影响神经突延伸。水凝胶的机械性能也显着影响神经突的延伸。在更柔韧的水凝胶上生长的 PC12 细胞表现出最大程度的神经突延伸。因此，PEG 水凝胶具有不同的生化和机械特性，可以增强神经再生。 (C) 2004 年 Wiley 期刊公司。
Photopolymerizable polyethylene glycol (PEG) hydrogels conjugated with bioactive ligands were examined for their use as scaffolds in peripheral nerve regeneration applications. The bioactivity and mechanical properties of PEG hydrogels can be tailored through the integration of bioactive factors (adhesion ligands, proteolytic sites, growth factors) and the alteration of PEG concentrations, respectively. For peripheral nerve regeneration, it will be important to determine the type and concentration of the bioactive molecules required to improve neurite extension. In this study, cell adhesion ligands (RGDS, IKVAV, and YIGSR) were covalently attached to PEG hydrogels. Both the type and concentration of cell adhesion ligand used affected neurite extension. Extension from PC12 cells was greater on hydrogels with RGDS incorporated than IKVAV, and the optimal concentration for each ligand was different. Cells adhered to but did not extend neurites on hydrogels with YIGSR. Cells did not adhere to hydrogels containing RGES. Furthermore, different combinations of these ligands affected neurite extension to different degrees. The mechanical properties of the hydrogels also significantly affected neurite extension. PC12 cells grown on more flexible hydrogels exhibited the greatest degree of neurite extension. PEG hydrogels have thus been developed with varying biochemical and mechanical properties that may enhance nerve regeneration. (C) 2004 Wiley Periodicals, Inc.