Effects of long-term estrogen replacement therapy on the prevalence and area of periarticular tibial osteophytes in surgically postmenopausal cynomolgus monkeys.
Effects of long-term estrogen replacement therapy on the prevalence and area of periarticular tibial osteophytes in surgically postmenopausal cynomolgus monkeys.
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DOI：
10.1016/j.bone.2007.04.175
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发表时间：
2007-08
期刊：
影响因子：
4.1
通讯作者：
E. J. Olson;B. Lindgren;C. Carlson
中科院分区：
文献类型：
--
作者：
E. J. Olson;B. Lindgren;C. Carlson
骨关节炎(OA)自然发生在食蟹猴身上。本研究的目的是：1)建立组织学测量方案，测量胫骨近端中轴和远轴骨赘的横截面积；2)确定长期雌激素替代治疗(ERT)对骨赘患病率和骨赘面积的影响；以及3)评估骨赘大小与骨关节炎危险因素(年龄和体重)以及合并的骨和软骨病变之间的关系。成年雌性食蟹猴(n=180)行双侧卵巢切除(OVX)，用ERT、大豆植物雌激素(SPE)或不加激素(OVX)治疗3年。在尸检时，对胫骨关节周围骨赘的患病率和横截面积进行组织学评估。分别用卡方分析和Kruskal-Wallis检验评价治疗对骨赘患病率和骨赘面积的影响；用回归分析评价其他比较。治疗组对胫骨内侧平台远轴骨赘的发生率无明显影响，但ERT组胫骨外侧平台远轴骨赘的发生率显著低于SPE组(p<；0.01)。ERT组远轴骨赘总数(内侧和外侧之和)明显低于OVX组和SPE组。两个胫骨平台的治疗对中轴型骨赘的发生率以及外侧型和内侧型中轴型骨赘的总和都没有显著影响。对两侧胫骨平台的轴向或远轴向骨赘横截面积无明显影响。胫骨平台外侧骨赘面积与胫骨内侧平台SCB厚度呈显著正相关(p=0.048)，而与年龄、体重无明显相关性。在这个自然发生的骨性关节炎模型中，长期的ERT并不能持续降低患病率，对胫骨关节周围骨赘的横截面积也没有显著影响。
Osteoarthritis (OA) occurs naturally in cynomolgus macaques. The purposes of the present study were to: 1) develop histological measurement schemes to measure the cross-sectional area of axial and abaxial osteophytes in the proximal tibia; 2) determine the effects of long-term estrogen replacement therapy (ERT) on osteophyte prevalence and area; and 3) assess relationships between osteophyte size and risk factors of OA (age and body weight) and concurrent bone and cartilage lesions. Adult female cynomolgus macaques (n=180) were bilaterally ovariectomized (OVX) and were treated for 3 years with ERT, soy phytoestrogens (SPE), or no hormones (OVX controls). At necropsy, the prevalence and cross-sectional area of periarticular tibial osteophytes were evaluated histologically. Treatment effects on osteophyte prevalence and area were evaluated using Chi-square analyses and Kruskal–Wallis test, respectively; other comparisons were evaluated using regression analyses. The prevalence of abaxial osteophytes in the medial tibial plateau was not significantly affected by treatment group; however, the prevalence of abaxial osteophytes in the lateral tibial plateau was significantly lower in ERT group than SPE group (p<0.01). The total number of abaxial osteophytes (sum of lateral and medial) was significantly lower in ERT group compared to OVX and SPE groups. Neither the prevalence of axial osteophytes nor the sum of lateral and medial axial osteophytes was significantly affected by treatment in either tibial plateau. There were no significant treatment effects on axial or abaxial osteophyte cross-sectional area in either tibial plateau. There was a significant positive correlation between abaxial osteophyte area and SCB thickness in the medial tibial plateau (p=0.048); however, there were no significant correlations between abaxial osteophyte area (medial or lateral) and age or body weight. In this model of naturally occurring OA, long-term ERT does not consistently reduce the prevalence, and has no significant effects on cross-sectional area, of periarticular tibial osteophytes.