Effect of enzymatic hydrolysis on cholesterol-lowering activity of oat β-glucan
Effect of enzymatic hydrolysis on cholesterol-lowering activity of oat β-glucan
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DOI：
10.1016/j.nbt.2009.11.003
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发表时间：
2010-02-28
影响因子：
5.4
通讯作者：
Lee, Hyeon Gyu
中科院分区：
文献类型：
--
作者：
Bae, In Young;Kim, Sung Mi;Lee, Hyeon Gyu
本研究采用酶法制备了燕麦β-葡聚糖水解物，其平均相对分子质量为730000 g/mol，具有较强的体外胆汁酸结合能力。此外，还在喂食高胆固醇饮食的大鼠身上评估了它的体内降胆固醇效果。补充β-葡聚糖水解物和天然β-葡聚糖可显著降低血清中的低密度脂蛋白和极低密度脂蛋白胆固醇水平，并进一步改善肝脏的脂质状况。当大鼠喂饲高胆固醇饲料并添加β-葡聚糖水解物时，观察到粪便胆汁酸排泄增加，这可能与体外胆汁酸结合能力有良好的相关性。此外，该水解物在增加粪便胆固醇和甘油三酯排泄方面比天然β-葡聚糖更有效，表明其在改善血脂方面是有效的。
In this study, oat beta-glucan hydrolysate, having average molecular weight of 730,000 g/mol which was previously shown to have great in vitro bile acid binding capacity, was prepared by enzymatic hydrolysis. Furthermore its in vivo hypocholestrolemic effects were evaluated in rats that were fed high-cholesterol diets. Supplements with beta-glucan hydrolysate as well as native beta-glucan significantly reduced the levels of LDL- and VLDL-cholesterol in serum and further improved the lipid profile in liver. When rats were fed high-cholesterol diets, supplemented with the beta-glucan hydrolysate, greater fecal bile acid excretion was observed, which could be favorably correlated to in vitro bile acid binding capacity. In addition, the hydrolysate was more effective at increasing the excretion of fecal cholesterol and triglyceride than the native beta-glucan, showing its effectiveness in improving the lipid profile.