Misoprostol induces relaxation of human corpus cavernosum smooth muscle: comparison to prostaglandin E1.
Misoprostol induces relaxation of human corpus cavernosum smooth muscle: comparison to prostaglandin E1.
复制标题
米索前列醇诱导人海绵体平滑肌松弛：与前列腺素 E1 的比较。
DOI：
10.1038/sj.ijir.3900497
复制
发表时间：
2000
影响因子：
2.6
通讯作者：
Traish,A
中科院分区：
文献类型：
--
作者：
Moreland,RB;Kim,NN;Nehra,A;Parulkar,BG;Traish,A
前列腺素 E 1 (PGE 1) 通过与人海绵体平滑肌上的特定 G 蛋白偶联受体相互作用并增加细胞内 cAMP 的合成来放松小梁平滑肌。米索前列醇 (Cytotec™) 是一种口服前列腺素 E 类似物。本研究的目的是比较米索前列醇与PGE 1 在人海绵体和培养的人海绵体平滑肌细胞中的功能活性。米索前列醇、米索前列醇游离酸或 PGE 1 在人海绵体条中诱导剂量依赖性松弛。当浓度大于 10−6 M 时，所有三种试剂均观察到组织再收缩。通过用血栓素 A2 受体拮抗剂 SQ29, 548 进行预处理，可以消除这种现象。根据这些观察，我们得出结论，米索前列醇被人海绵体原位激活，并在体外松弛去氧肾上腺素预浓缩的组织条。这种松弛反应是由这些药物增加的 cAMP 合成介导的。
Prostaglandin E 1 (PGE 1) relaxes trabecular smooth muscle by interacting with specific G-protein coupled receptors on human corpus cavernosum smooth muscle and increasing intracellular synthesis of cAMP. Misoprostol (Cytotec™), is an oral prostaglandin E analogue. The purpose of this study was to compare the functional activity of misoprostol with PGE 1 in human corpus cavernosum and cultured human corpus cavernosum smooth muscle cells. Misoprostol, misoprostol free acid or PGE 1 induced dose-dependent relaxations in strips of human corpus cavernosum. At concentrations greater than 10− 6 M, tissue recontraction was observed with all three agents. This was abrogated by pretreatment with the thromboxane A2 receptor antagonist SQ29, 548. From these observations, we conclude that misoprostol is activated by human corpus cavernosum in situ and relaxes phenylephrine-precontrated tissue strips in vitro. This relaxation response is mediated by the increased cAMP synthesis by these agents.