Expression of a luxS gene is not required for Borrelia burgdorferi infection of mice via needle inoculation
Expression of a luxS gene is not required for Borrelia burgdorferi infection of mice via needle inoculation
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DOI：
10.1128/iai.71.5.2892-2896.2003
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发表时间：
2003-05-01
影响因子：
3.1
通讯作者：
Norgard, MV
中科院分区：
文献类型：
--
作者：
Hübner, A;Revel, AT;Norgard, MV
luxS基因产物是细菌中LuxS/自诱导物-2（AI-2）群体感应系统的组成部分。一个假定的luxS基因表达水平相当的伯氏疏螺旋体菌株297培养无论是在体外或在透析膜室植入大鼠腹膜腔。虽然疏螺旋体luxS基因在功能上补充了大肠杆菌DH 5 α中的LuxS缺陷，但在B中不能检测到Al-2样活性。Burgdorferi培养物上清液或浓缩的细胞裂解物。最后，获得了B的luxS缺陷型突变体。当将burgdorferi皮内针接种到小鼠中时，其在野生型水平具有感染性，表明luxS的表达可能不是感染性所需的，但至少不是哺乳动物宿主适应所必需的。我们的发现也挑战了LuxS/AI-2群体感应系统在B中起作用的概念。burgdorferi。
The luxS gene product is an integral component of LuxS/autoinducer-2 (AI-2) quorum-sensing systems in bacteria. A putative luxS gene was expressed at comparable levels by Borrelia burgdorferi strain 297 cultivated either in vitro or in dialysis membrane chambers implanted in rat peritoneal cavities. Although the borrelial luxS gene functionally complemented a LuxS deficiency in Escherichia coli DH5alpha, Al-2-like activity could not be detected within B. burgdorferi culture supernatants or concentrated cell lysates. Finally, a luxS-deficient mutant of B. burgdorferi was infectious at wild-type levels when it was intradermally needle inoculated into mice, indicating that expression of luxS probably is not required for infectivity but, at the very least, is not essential for mammalian host adaptation. Our findings also challenge the notion that a LuxS/AI-2 quorum-sensing system is operative in B. burgdorferi.