Detection of mitochondrial genome depletion by a novel cDNA in renal cell carcinoma.
Detection of mitochondrial genome depletion by a novel cDNA in renal cell carcinoma.
复制标题
通过肾细胞癌中的新型 cDNA 检测线粒体基因组缺失。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
Srinivasan Rajaraman
中科院分区：
文献类型：
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作者：
Peter Selvanayagam;Srinivasan Rajaraman
通过消减杂交程序分离的 cDNA 在从肾细胞癌患者获得的 13 个肿瘤肾组织中的 8 个中检测到 mtDNA 和编码 NADH 脱氢酶亚基 3 的 mRNA 的丢失。测序揭示了一段与 cDNA 中部线粒体 NADH 脱氢酶亚基 3 基因同源的核苷酸。在六种肾癌细胞系中的五种中也观察到了耗尽现象。然而，在良性肾嗜酸细胞瘤的情况下，mtDNA 含量比邻近正常组织增加了 200%。这种现象在肾细胞癌中发生的频率很高，但在其他类型的癌症中则不然，表明这可能是与肾细胞肿瘤转化相关的重要表型。然而，线粒体基因组内不存在任何结构改变表明，这种消耗可能是与致癌转化过程相关的继发事件。
A cDNA isolated by a subtractive hybridization procedure detected loss of mtDNA and the mRNA coding for NADH dehydrogenase subunit 3 in 8 of 13 tumor kidney tissues obtained from patients with renal cell carcinoma. Sequencing revealed a stretch of nucleotides homologous to the mitochondrial NADH dehydrogenase subunit 3 gene in the middle of the cDNA. The depletion phenomenon was also observed in five of six renal carcinoma cell lines. In the case of a benign renal oncocytoma, however, the mtDNA content was increased 200% more than that of the adjacent normal tissue. The frequency with which this phenomenon occurs in renal cell carcinomas, but not in other types of cancers, suggests that this may be an important phenotype associated with renal cell neoplastic transformation. However, the absence of any structural alterations within the mitochondrial genome suggests that the depletion may be a secondary event associated with the oncogenic transformation process.