Micellar electrokinetic chromatography: Methodological and instrumental advances focused on practical aspects
Micellar electrokinetic chromatography: Methodological and instrumental advances focused on practical aspects
复制标题
胶束电动色谱：方法学和仪器的进步侧重于实际方面
作者：
Manuel Silva
本文回顾了最新的方法和仪器的改进，以提高灵敏度和分辨率在MEKC‐为基础的测定。在线样品浓度（堆叠、扫描和两种方案的组合）和其他毛细管上方法，如毛细管衍生化和流动注射系统与MEKC的耦合，是讨论的提高灵敏度的最相关的方法学方法。同时，通过使用有机改性剂、离子液体、阳离子、CDs、非离子和两性离子表面活性剂、混合胶束、囊泡和碳纳米结构以及2 - D CE和化学计量技术来提高分辨率，也详细考虑了BGE的变化。本文还讨论了两种仪器方法，如质谱和LIF，包括克服MEKC与质谱直接耦合所带来的困难的建议，以及LIF使用典型的氩离子激光器和最近使用二极管激光器作为激励源所取得的优异检测灵敏度。文章还对未来的发展方向提出了一些看法。
This article reviews the latest methodological and instrumental improvements for enhancing sensitivity and resolution in MEKC‐based determinations. On‐line sample concentration (stacking, sweeping and combination of two protocols) and other on‐capillary approaches such as in‐capillary derivatization and the coupling of flow‐injection systems with MEKC are the most relevant methodological approaches discussed for improving sensitivity. At the same time, changes in the BGE through the use of organic modifiers, ionic liquids, cations, CDs, non‐ionic and zwitterionic surfactants, mixed micelles, vesicles and carbon nanostructures as well as 2‐D CE and chemometric techniques for enhancing resolution are also considered in detail. Two instrumental approaches such as MS and LIF are also discussed, covering the proposals for overcoming the difficulties arising from the direct coupling of MEKC with MS and the excellent detection sensitivity achieved by LIF using the typical argon‐ion laser and recent growth in the use of diode lasers as excitation sources. Some thoughts on potential future directions are also expressed.
影响因子：
7.4
作者：
Zhu, Cuiru;He, Xinya;Dovichi, Norman J.
通讯作者：
Dovichi, Norman J.