Inferring the mycorrhizal status of introduced plants of Cypripedium calceolus (Orchidaceae) in northern England using stable isotope analysis
Inferring the mycorrhizal status of introduced plants of Cypripedium calceolus (Orchidaceae) in northern England using stable isotope analysis
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DOI：
10.1093/botlinnean/box104
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发表时间：
2018-04-01
影响因子：
2.4
通讯作者：
Gebauer, Gerhard
中科院分区：
文献类型：
--
作者：
Fay, Michael F.;Feustel, Moritz;Gebauer, Gerhard
作为在英格兰的Cypripedium calceolus的保护计划的一部分，幼苗已产生在无菌培养和盆栽种植之前，在pupilium适合的位置在英格兰北方，这些引进的成功预计将取决于适当的菌根真菌的存在下，在引进网站和重新建立菌根协会的引进植物。为了研究菌根的功能，采用盆栽方法对C.从天然野生地点和三个引入地点的植物中收集蒲包花属和相关的非兰花物种，并筛选碳（C）、氮（N）和氢（H）同位素的富集，所述同位素指示真菌和兰花之间在兰花菌根联合体中的物质交换。还测量了总氮浓度。在所有地点，C.与伴生种相比，盾叶薯蓣富含H-2，N-15含量较低，总N含量较高。C-13没有明显的富集。总之，这些结果表明，所有筛选的C。calceolus（野生的和引进的）可能与丝核菌形成了菌根联合体。因此，在引入后，无菌产生的幼苗可能已经与C.在其分布区的其他地方。
As part of the conservation programme for Cypripedium calceolus in England, seedlings have been produced in axenic culture and grown in pots prior to planting out at putatively suitable locations in northern England; the success of these introductions is expected to be dependent on the presence of appropriate mycorrhizal fungi at the introduction sites and the re-establishment of mycorrhizal associations by the introduced plants. To investigate mycorrhizal function, leaf samples of C. calceolus and associated non-orchid species were collected from the native wild site and from plants at three of the introduction sites and screened for enrichment in isotopes of carbon (C), nitrogen (N) and hydrogen (H) that are indicative of an exchange of matter between fungi and orchids in orchid mycorrhizal associations. Total N concentration was also measured. At all sites, plants of C. calceolus were enriched in H-2 and, to a lesser extent, N-15 relative to the associated species and had higher concentrations of total N. There was no apparent enrichment in C-13. Together, these results indicate that all the screened individuals of C. calceolus (wild and introduced) are likely to have formed a mycorrhizal association with a rhizoctonia. Thus, after introduction the axenically produced seedlings may have established a mycorrhizal relationship with the same type of fungus utilized by C. calceolus elsewhere in its range.