Hydrostatic pressure regulates tight junctions, actin cytoskeleton and transcellular ion transport.
Hydrostatic pressure regulates tight junctions, actin cytoskeleton and transcellular ion transport.
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DOI：
10.1016/j.bbrc.2009.10.144
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发表时间：
2009-12
影响因子：
3.1
通讯作者：
Shinsaku Tokuda;H. Miyazaki;K. Nakajima;Toshiki Yamada;Y. Marunaka
中科院分区：
文献类型：
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作者：
Shinsaku Tokuda;H. Miyazaki;K. Nakajima;Toshiki Yamada;Y. Marunaka
在上皮细胞和内皮细胞中，紧密连接通过细胞旁途径调节几种物质的运动，维持顶端和基底室之间的几个梯度，包括渗透压和静水压力。在这项研究中，我们发现静水压力梯度的改变影响紧密连接以及肌动蛋白细胞骨架、细胞高度和跨细胞离子运输。从基底侧到顶端的静水压力梯度在几十分钟内增加了跨上皮电导并改变了claudin-1的定位。通过消除静水压力梯度，这些变化迅速恢复。静水压力梯度还引起肌动蛋白结构和细胞高度的动态变化。我们进一步发现，从基底侧到顶侧的静水压力梯度刺激了跨细胞的CL-−转运。我们目前的发现表明，上皮细胞的结构和功能受到由上皮细胞自身产生和维持的流体静压梯度的调节。
In the epithelia and endothelia, tight junctions regulate the movement of several substances through the paracellular pathway, maintaining several gradients between apical and basal compartments including osmolality and hydrostatic pressure. In this study, we show that the change of hydrostatic pressure gradient affected tight junctions as well as actin cytoskeleton, cell height and transcellular ion transport. Hydrostatic pressure gradient from basolateral to apical side increased transepithelial conductance and altered claudin-1 localization within several tens of minutes. These changes were promptly restored by the elimination of hydrostatic pressure gradient. Hydrostatic pressure gradient also induced dynamic changes in the actin structure and cell height. We further found that hydrostatic pressure gradient from basolateral to apical side stimulates transcellular Cl−transport. Our present findings indicate that the epithelial cell structures and functions are regulated by the hydrostatic pressure gradient which is generated and maintained by the epithelia themselves.