Modular Chamber Assembled with Cell-Replicated Surface for Capture of Cancer Cells
Modular Chamber Assembled with Cell-Replicated Surface for Capture of Cancer Cells
复制标题
组装有细胞复制表面的模块化室用于捕获癌细胞
DOI：
10.1021/acsbiomaterials.8b01605
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发表时间：
2019
影响因子：
--
通讯作者：
Jia Lingyun
中科院分区：
文献类型：
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作者：
Sun He;Hang Lulu;Yang Liwei;Yang Yan;Jiang Wenning;Xu Ting;Jia Lingyun
循环肿瘤细胞（CTC）的捕获主要是使用磁性纳米颗粒和复杂的微流体用少量血液进行的。在这项研究中，我们提出了一个CTC捕获装置的基础上，模块化设计，并呼吁该装置作为CTC室。与其他CTC捕获装置不同，CTC室的容量可以通过改变CTC捕获模块的数量来改变，以容纳不同体积的血液样品。CTC捕获模块的核心组件是具有细胞复制拓扑结构和抗EpCAM抗体涂层的聚二甲基硅氧烷（PDMS）膜。这两种协同作用可以提高癌细胞的捕获率。此外，CTC腔室与一个或三个CTC捕获模块组装，用于从代表晚期（3 mL血液，10个癌细胞mL-1）或中早期（9 mL血液，1个癌细胞mL-1）癌症的掺入血液样品中捕获癌细胞。结果表明，在1 h内可获得较高的捕获率（EpCAM阳性，约80%; EpCAM阴性，约65%）和纯度（EpCAM阳性，约90%; EpCAM阴性，约80%）。因此，这种经济和简便的CTC室可以为设计适用于诊断不同阶段癌症的下一代CTC检测设备提供机会。
The capture of circulating tumor cells (CTCs) is mainly carried out with a small volume of blood using magnetic nanoparticles and complex microfluidics. In this study, we propose a CTC-capture apparatus based on a modular design and called this apparatus as the CTC chamber. Distinct from other CTC-capture apparatuses, the capacity of the CTC chamber could be altered by varying the number of CTC-capture modules to accommodate the different volumes of blood sample. The core component of the CTC-capture module was a polydimethylsiloxane (PDMS) film with cell-replicated topological structure and anti-EpCAM antibody coating. Both synergistic roles can enhance the capture yield of cancer cells. Furthermore, the CTC chamber was assembled with one or three CTC-capture modules for the capture of cancer cells from spiked blood samples representing late-stage (3 mL of blood, 10 cancer cells mL–1) or middle-early stage (9 mL of blood, 1 cancer cell mL–1) cancer. The results showed that high capture yield (EpCAM-positive, ∼80%; EpCAM-negative, ∼65%) and purity (EpCAM-positive, ∼90%; EpCAM-negative, ∼80%) could be obtained within 1 h. This economic and facile CTC chamber could therefore open up opportunities for designing the next-generation CTC detection devices suitable for the diagnosis of different stages of cancer.