Protein Synthesis during Germination and Appressorium Formation of Colletotrichum lagenarium Spores
Protein Synthesis during Germination and Appressorium Formation of Colletotrichum lagenarium Spores
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炭疽菌孢子萌发和附着胞形成过程中的蛋白质合成
DOI：
10.1099/00221287-124-1-61
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发表时间：
1981
期刊：
影响因子：
1.5
通讯作者：
M. Yamamoto
中科院分区：
文献类型：
--
作者：
K. Suzuki;I. Furusawa;N. Ishida;M. Yamamoto
总结：采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和荧光光谱法研究了西瓜炭疽菌（Colletotrichum lagenarium）孢子萌发和附着胞形成过程中蛋白质的合成。在培养的早期阶段（5- 25分钟），仅在孢子匀浆的20000 g上清液部分中检测到分子量为72、43和38千道尔顿的多肽的合成。这些多肽可能与孢子萌发所必需的蛋白质合成有关，孢子萌发发生在孵育开始后40分钟内。只有当附着胞形成时，才特异性地合成分子量为95千道尔顿的多肽。当蛋白质的合成，包括95千道尔顿的多肽，被完全抑制放线菌酮加入孵育1小时后，附着胞成熟的结构，但没有功能，他们似乎没有能力穿透人工膜。
SUMMARY: Protein synthesis during germination and appressorium formation of Colletotrichum lagenarium spores was investigated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and fluorography. Synthesis of polypeptides with molecular masses of 72, 43 and 38 kilodaltons was detected only in the 20000 g supernatant fraction of spore homogenates at early stages (5--25 min) of incubation. These polypeptides are probably associated with the protein synthesis essential for spore germination that occurs within 40 min from the start of incubation. A polypeptide with a molecular mass of 95 kilodaltons was specifically synthesized only when appressoria were formed. When synthesis of proteins, including the 95 kilodalton polypeptide, was completely inhibited by cycloheximide added after 1 h incubation, appressoria matured in structure but not in function; they seemed to have no ability to penetrate artificial membranes.