NUCLEAR-MAGNETIC-RESONANCE (NMR) IMAGING OF IRON OXIDE-LABELED NEURAL TRANSPLANTS
NUCLEAR-MAGNETIC-RESONANCE (NMR) IMAGING OF IRON OXIDE-LABELED NEURAL TRANSPLANTS
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DOI：
10.1006/exnr.1993.1085
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发表时间：
1993-06-01
影响因子：
5.3
通讯作者：
LAUTERBUR, PC
中科院分区：
文献类型：
--
作者：
HAWRYLAK, N;GHOSH, P;LAUTERBUR, PC
使用成年大鼠脑体内核磁共振（NMR）成像来观察氧化铁标记的脑内神经移植物的命运。宿主动物接受胎儿（E17-E18）大鼠组织的移植物，其制备为细胞悬浮液，并通过与含有氧化铁颗粒的重构仙台病毒包膜一起孵育来标记。在仅进行手术创伤或移植未标记细胞悬浮液后的动物中进行对照研究。在术后5至60天之间对每只动物进行一次体内NMR成像（二维傅里叶变换或三维傅里叶变换）。含有标记细胞的宿主大脑的核磁共振图像显示了足够的解剖细节，可用于识别主要大脑结构。在 T2 加权 NMR 图像中，移植部位被视为皮质中的暗区（低强度信号）。三价铁的组织化学染色（普鲁士蓝染色）表明，在与 NMR 图像中的暗区相对应的组织切片中存在大量普鲁士蓝阳性细胞。移植后10天存活的具有神经元样形态的普鲁士蓝阳性细胞的数量比移植后1-2个月时相对较多。含有标记细胞的宿主的 NMR 图像以及免疫组织化学和组织化学染色表明星形胶质细胞和巨噬细胞的细胞迁移距离移植部位最远 2 毫米。迁移细胞主要沿着宿主白质中的纤维束移动。对照动物的大脑中也存在普鲁士蓝沉积物，但同一动物的核磁共振图像并不像接受标记细胞移植的大脑图像中那样包含明显的暗区。这些结果表明核磁共振成像可用于研究神经移植手术后的细胞存活和迁移。
Nuclear magnetic resonance (NMR) imagingin vivoof adult rat brains was used to observe the fate of iron oxide-labeled intracerebral neural grafts. The host animals received grafts of fetal (E17-E18) rat tissue prepared as cell suspensions and labeled by incubation with reconstituted Sendai viral envelopes containing iron oxide particles. Control studies were performed in animals following surgical trauma alone or transplantation of unlabeled cell suspensions.In vivoNMR imaging (either two-dimensional Fourier transform or three-dimensional Fourier transform) was performed once on each animal between 5 and 60 days postsurgery. The NMR images of the host brains containing the labeled cells showed sufficient anatomical detail for the identification of the major brain structures. The graft sites were seen in the T2-weighted NMR images as dark regions (low-intensity signal) in the cortex. Histochemical staining for ferric iron (prussian blue stain) demonstrated the presence of numerous prussian blue-positive cells in tissue sections corresponding to the dark regions in the NMR images. Surviving prussian blue-positive cells with neuron-like morphology were relatively more numerous 10 days after transplantation than at 1-2 months postgrafting. The NMR images and immunohistochemical and histochemical staining of hosts containing labeled cells suggest cell migration of astrocytes and macrophages up to 2 mm away from the graft sites. The migrating cells were primarily along fiber tracts in the host white matter. Prussian blue deposits were also present within the brains of the control animals, but the NMR images from the same animals did not contain distinct dark regions like those in the images of brains which had received labeled cell transplants. These results demonstrate that NMR imaging can be used to study cell survival and migration following neural grafting procedures.