Fabrication and characteristics of chitosan sponge as a tissue engineering scaffold.
Fabrication and characteristics of chitosan sponge as a tissue engineering scaffold.
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DOI：
10.1155/2014/786892
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发表时间：
2014
影响因子：
--
通讯作者：
Hayashi Y
中科院分区：
文献类型：
--
作者：
Ikeda T;Ikeda K;Yamamoto K;Ishizaki H;Yoshizawa Y;Yanagiguchi K;Yamada S;Hayashi Y
细胞、生长因子和支架是创建组织工程结构所需的三个主要因素。牛海绵状脑病（BSE）出现后，相当多的注意力因此集中在非牛材料。在这项研究中，我们研究了壳聚糖多孔支架的性能。对不同浓度的壳聚糖溶液进行控制冷冻和冻干，制备了多孔壳聚糖海绵。采用扫描电镜对材料进行了检测，并对壳聚糖海绵的孔隙率、拉伸强度和碱性成纤维细胞生长因子（bFGF）的体外释放进行了研究。壳聚糖支架的形貌为典型的微孔结构，孔径在50 ~ 200 μm之间。不同浓度壳聚糖支架的孔隙率在75 ~ 85%之间。随着壳聚糖浓度的增加，孔隙率呈下降趋势。拉伸性能与壳聚糖浓度的关系表明，壳聚糖浓度越高，海绵的极限拉伸强度越高。体外释放研究表明，壳聚糖溶液浓度越高，壳聚糖海绵中bFGF的释放时间越长。
Cells, growth factors, and scaffolds are the three main factors required to create a tissue-engineered construct. After the appearance of bovine spongiform encephalopathy (BSE), considerable attention has therefore been focused on nonbovine materials. In this study, we examined the properties of a chitosan porous scaffold. A porous chitosan sponge was prepared by the controlled freezing and lyophilization of different concentrations of chitosan solutions. The materials were examined by scanning electron microscopy, and the porosity, tensile strength, and basic fibroblast growth factor (bFGF) release profiles from chitosan sponge were examined in vitro. The morphology of the chitosan scaffolds presented a typical microporous structure, with the pore size ranging from 50 to 200 μm. The porosity of chitosan scaffolds with different concentrations was approximately 75–85%. A decreasing tendency for porosity was observed as the concentration of the chitosan increased. The relationship between the tensile properties and chitosan concentration indicated that the ultimate tensile strength for the sponge increased with a higher concentration. The in vitro bFGF release study showed that the higher the concentration of chitosan solution became, the longer the releasing time of the bFGF from the chitosan sponge was.