The presence of halide salts influences the non-covalent interaction of MRI contrast agents and human serum albumin
The presence of halide salts influences the non-covalent interaction of MRI contrast agents and human serum albumin
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DOI：
10.1002/cmmi.153
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发表时间：
2007-09-01
影响因子：
--
通讯作者：
Elst, Luce Vander
中科院分区：
文献类型：
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作者：
Henrotte, Virginie;Muller, Robert N.;Elst, Luce Vander
本研究的基本原理和目的是评价实验条件（缓冲液、盐等）的影响。基于表征MRI造影剂和人血清白蛋白之间的非共价相互作用以及因此它们的体内弛豫率的数据。通过测量不同实验条件下质子弛豫速率增强，评价了三种钆对比剂（Gd-EOB-DTPA、Gd-BOPTA和MP-2269）与人血清白蛋白的相互作用。数据显示卤化物盐对三种造影剂的顺磁弛豫增强的负面影响。因此，卤化物盐的存在可能对与HSA相互作用的MRI造影剂的功效具有负面影响。另外，必须仔细注意不同研究中报道的各种造影剂的结合参数的比较，因为介质的组成可以极大地影响非共价相互作用。版权所有（C）2007约翰威利父子有限公司
The rationale and objectives of the study were to evaluate the influence of the experimental conditions (buffer, salt, etc.) on the data characterizing the non-covalent interaction between MRI contrast agents and human serum albumin and hence their in vivo relaxivity. The interaction of three gadolinium contrast agents (Gd-EOB-DTPA, Gd-BOPTA and MP-2269) with human serum albumin was assessed through the measurement of proton relaxation rate enhancement in various experimental conditions. The data show the negative effect of halide salts on the paramagnetic relaxation enhancement of the three contrast agents. The presence of halide salts can thus have a negative effect on the efficacy of MRI contrast agents interacting with HSA. In addition., careful attention must be paid to comparisons of the binding parameters of various contrast agents reported in different studies since the composition of the medium can greatly influence the non-covalent interaction. Copyright (C) 2007 John Wiley & Sons, Ltd.