Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin
Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin
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DOI：
10.5511/plantbiotechnology.10.0610a
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发表时间：
2010-01-01
影响因子：
1.6
通讯作者：
Tanaka, Yoshikazu
中科院分区：
文献类型：
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作者：
Nakamura, Noriko;Fukuchi-Mizutani, Masako;Tanaka, Yoshikazu
通过内源类黄酮 3'-羟化酶 (F3'H) 和类黄酮 3', 5'-羟化酶 (F3'5'H) 基因的下调与异源基因的表达相结合，从蓝色或紫色品种中获得了具有各种粉红色花瓣色调的转基因蓝猪耳植物。蓝色蓝猪草品种中 F3'H 和 F3'5'H 基因的敲低通过降低花青素和飞燕草素水平并增加基于天竺葵素的花青素水平而产生淡粉色系。玫瑰二氢黄酮醇 4-还原酶 (DFR) 基因的额外表达提高了天竺葵素的水平，并产生了深粉色的花瓣。天竺葵素DFR基因的表达代替玫瑰DFR基因的表达增加了天竺葵素的水平并使花瓣颜色变深。将含有 DFR 基因的两种基因构建体引入紫色 torenia 品种中，该品种比蓝色品种具有更多的花青素和更深的颜色，进一步提高了天竺葵素的水平和粉红色的强度。这些结果表明，合适的基因源和宿主的选择极大地影响所得转基因植物的表型。
Transgenic torenia plants with various pink petal tones were obtained from blue or violet cultivars by combining downregulation of endogenous flavonoid 3'-hydroxylase (F3'H) and flavonoid 3', 5'-hydroxylase (F3'5'H) genes with expression of a heterologous gene. Knockdown of the F3'H and F3'5'H genes in a blue torenia cultivar resulted in pale pink lines by decreasing the cyanidin and delphinidin levels and increasing the pelargonidin-based anthocyanin levels. Additional expression of rose dihydroflavonol 4-reductase (DFR) gene elevated the level of pelargonidin and yielded darker pink petals. Expression of pelargonidin DFR gene instead of the rose DFR gene increased the level of pelargonidin and darkened the petal color. Introducing the two genetic constructs containing a DFR gene into a violet torenia cultivar, which had more anthocyanins and a darker color than the blue one, further increased the level of pelargonidin and pink color intensity. These results reveal that selection of a suitable gene source and host greatly affects the phenotypes of the resultant transgenic plants.