Energy-dependent redox state of heme a + a 3 and copper of cytochrome oxidase in perfused rat brain in situ.
Energy-dependent redox state of heme a + a 3 and copper of cytochrome oxidase in perfused rat brain in situ.
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原位灌注大鼠脑中血红素 a a 3 和细胞色素氧化酶铜的能量依赖性氧化还原状态。
DOI：
10.1152/ajpcell.1998.275.4.c1022
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
N. Yoshimura
中科院分区：
文献类型：
--
作者：
A. Matsunaga;Y. Nomura;S. Kuroda;M. Tamura;J. Nishihira†;N. Yoshimura
利用无血灌注大鼠脑，研究了血红素a + a3和铜两种发色团细胞色素氧化酶的氧化还原行为。当停止灌注流诱导全脑缺血时，血红素a + a3的减少呈快速、缓慢和第二快速的三阶段。血红素a + a3快速相后，铜的还原为单相。血红素a + a3的三相还原被能量消耗处理（如添加解偶联剂）所减弱。铜的还原时间过程不受能量损耗的影响。在全面缺血期间，磷酸肌酸的减少几乎与血红素a + a3的减少平行，而ATP保持在对照水平，直到快速阶段血红素a + a3减少约60%。在缓慢阶段，ATP开始随着铜的还原而减少。铜的氧化还原行为与血红素a+ a3还原的慢相相似，这是因为铜比血红素a+ a3具有更高的氧亲和力。因此，血红素a + a3的快速还原阶段可以作为ATP减少前的警报，而铜的还原表明在严重缺氧下ATP减少。因此，铜信号在无创近红外光谱是一个有用的参数，为临床设置。
Using the blood-free perfused rat brain, we examined the redox behavior of cytochrome oxidase of two chromophores, heme a + a 3 and copper. When perfusate inflow was stopped to induce global ischemia, the reduction of heme a + a 3 was triphasic, with a rapid phase, a slow phase, and a second rapid phase. In contrast, the reduction of copper was monophasic after the rapid phase of heme a + a 3. The triphasic reduction of heme a + a 3 was diminished by energy-depleting treatments, such as addition of an uncoupler. The time course of the reduction of copper was not affected by the energy depletion. During global ischemia the decrease in creatine phosphate nearly paralleled the reduction of heme a + a 3, whereas ATP remained at the control level until ∼60% of heme a + a 3 was reduced in the rapid phase. In the slow phase, ATP started to decrease with the reduction of copper. The redox behavior of copper was similar to the slow phase of the reduction of heme a+ a 3 because of the higher oxygen affinity of copper than of heme a + a 3. Therefore, the rapid phase of the reduction of heme a + a 3 can be used as an alarm before a decrease in ATP, whereas the reduction of copper indicates a decrease in ATP under severe hypoxia. Thus the copper signal in noninvasive near-infrared spectroscopy is a useful parameter for the clinical setting.
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对（羟基汞）苯甲酸钠对细胞色素 C 氧化酶中 CuA 中心的化学修饰。
影响因子：
2.9
作者：
Gelles,J;Chan,SI
通讯作者：
Chan,SI
常氧和缺氧条件下有血脑和无血脑中细胞色素氧化酶血红素铁和铜吸收的检测。
影响因子：
2.9
作者：
Miyake,H;Nioka,S;Zaman,A;Smith,DS;Chance,B
通讯作者：
Chance,B
氟碳换血后缺氧引起的猫脑细胞色素c氧化酶氧化还原变化。
DOI：
10.1152/ajpheart.1995.269.2.h417
发表时间：
1995
期刊：
The American journal of physiology
影响因子：
--
作者：
Ferrari,M;Williams,MA;Wilson,DA;Thakor,NV;Traystman,RJ;Hanley,DF
通讯作者：
Hanley,DF
在氟碳灌注的猫中，细胞色素 C 氧化酶铜的减少先于脑部 O2 利用失败。
DOI：
10.1152/ajpheart.1996.271.2.h579
发表时间：
1996
期刊：
The American journal of physiology
影响因子：
--
作者：
Stingele,R;Wagner,B;Kameneva,MV;Williams,MA;Wilson,DA;Thakor,NV;Traystman,RJ;Hanley,DF
通讯作者：
Hanley,DF