T lymphocytes can recognize determinants unique to neuropeptides of guinea pig myelin basic protein containing a single D-isomer amino acid substitution.
T lymphocytes can recognize determinants unique to neuropeptides of guinea pig myelin basic protein containing a single D-isomer amino acid substitution.
复制标题
T淋巴细胞可以识别含有单个D-异构体氨基酸取代的豚鼠髓磷脂碱性蛋白的神经肽所特有的决定簇。
DOI：
10.1002/jnr.490250105
复制
发表时间：
1990
影响因子：
4.2
通讯作者：
Man,EH
中科院分区：
文献类型：
--
作者：
Levy,RB;Miller,S;Garcia,NM;Jones,M;Fisher,GH;Man,EH
本研究旨在研究豚鼠髓鞘碱性蛋白（GPMBP）合成肽中选定氨基酸消旋形式的替代如何影响宿主识别这些分子的免疫能力。利用GPMBP 69-84序列中含有aD -丝氨酸在70或75 （69-84 [D‐ser70orD‐ser75]）和aD -天冬氨酸在82 （69-84 [D‐asp82]）的肽，研究结果表明，这些神经肽上非对映体取代的位置对免疫系统识别该分子的能力至关重要。因此，在82位替换D - asp或在75位替换D - ser，可以消除这些肽诱导实验性自身免疫性脑炎和宿主T细胞增殖的能力。相比之下，含有D‐ser70残基的肽能够在大鼠中诱导临床疾病，并刺激69-84‐（D‐ser70）‐注射动物的T淋巴细胞。此外，尽管该D -肽被证明至少具有相同的决定因子刺激细胞系，但该分子特有的一些表位可以刺激CD4+同基因T细胞。
The present studies were undertaken to examine how the substitution of racemized forms of selected amino acids in synthetic peptides of guinea pig myelin basic protein (GPMBP) would after the host's immunological ability to recognize such molecules. Using peptides from the 69–84 sequence of GPMBP containing aD‐serine at position 70 or 75 (69–84[D‐ser70orD‐ser75]) orD‐asparate at position 82 (69–84[D‐asp82]), the findings demonstrated that the position of the diastereomer substitution on tthese neuropeptides was critical with respect to the ability of the immune system to recognize the molecule. Thus substitution of D‐asp at position 82 or D‐ser at position 75 abrogated the ability of these peptides to induce experimental autoimmune encephalitis and proliferation of host T cells. In contrast, a peptide containing a D‐ser70residue was capable of inducing clinical disease in rats, as well as stimulating T lymphocytes from 69–84‐(D‐ser70)‐injected animals. Moreover, although this D‐peptide was shown to share at least determinant(s)‐stimulated cell lines demonstrated that some epitope(s) unique to this molecule could stimulate CD4+syngeneic T cells.