Induction of insulin-like growth factor 1 splice forms by subfragments of myofibrillar proteins
Induction of insulin-like growth factor 1 splice forms by subfragments of myofibrillar proteins
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DOI：
10.1016/j.mce.2014.08.010
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发表时间：
2015-01-05
影响因子：
4.1
通讯作者：
Popov, Vladimir O.
中科院分区：
文献类型：
--
作者：
Kravchenko, Irina V.;Furalyov, Vladimir A.;Popov, Vladimir O.
胰岛素样生长因子-1(IGF-1)mRNAs剪接形式的表达最近被证明是由受损肌肉释放的肌原纤维蛋白刺激的。在这项研究中，我们报道了由FN III型结构域和Ig样结构域组成的肌蛋白和肌球蛋白的单个亚片段可以在蛋白质和mRNA水平上激活培养的成肌细胞中两种IGF-1剪接形式的表达。竞争研究表明，每种结构域类型都与自己的受体相互作用。不同类型结构域诱导的IGF-1表达对调节级联抑制物的敏感性不同。FNⅢ结构域对钙/钙调蛋白依赖性蛋白激酶的抑制更为敏感，而类Ig结构域对腺苷环化酶-cAMP-PKA通路的抑制更为敏感。(C)2014爱思唯尔爱尔兰有限公司。保留所有权利。
Expression of insulin-like growth factor 1 (IGF-1) mRNAs splice forms was recently shown to be stimulated by myofibrillar proteins released from the damaged muscle. In this study, we report that individual subfragments of titin and myomesin composed of Fn type III and Ig-like domains can activate expression of two IGF-1 splice forms in cultured myoblasts, both at protein and mRNA levels. Competition studies showed that each of the domain-types interacts with its own receptor. Induction of IGF-1 expression caused by domains of different types showed dissimilar sensitivity to inhibitors of regulatory cascades. The effect of Fn type III domains was more sensitive to inhibition of Ca2+/calmodulin dependent protein kinase, whereas the effect of Ig-like domains showed greater sensitivity to the inhibition of the adenylyl cyclase-cAMP-PKA pathway. (C) 2014 Elsevier Ireland Ltd. All rights reserved.