INCREASE IN RATIO OF 18S RNA TO 28S RNA IN CYTOPLASM OF MOUSE TISSUES DURING AGING
INCREASE IN RATIO OF 18S RNA TO 28S RNA IN CYTOPLASM OF MOUSE TISSUES DURING AGING
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DOI：
10.1016/0047-6374(78)90029-5
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发表时间：
1978-01-01
影响因子：
5.3
通讯作者：
GOTO, S
中科院分区：
文献类型：
--
作者：
MORI, N;MIZUNO, D;GOTO, S
本文研究了衰老过程中小鼠肝、肾和脑细胞质RNA变化的可能性。在蔗糖梯度离心中，这些器官的胞质RNA在260 nm处的光密度曲线相似，在28S、18S和4S处有3个主峰。脑和肝中18S和28S RNA的比例随年龄的增长而显著增加。无论动物年龄如何，在天然和变性条件下，3种组织提取物的聚丙烯酰胺凝胶电泳图谱几乎相同。由于凝胶上分离的大多数次要组分可能是rRNA的体内降解产物，因此rRNA中明显和隐藏断裂的程度在老化过程中显然没有变化。
The possibility of alterations in cytoplasmic RNA in mouse liver, kidney and brain during aging was investigated. The cytoplasmic RNA in these organs gave similar profiles of optical density at 260 nm with 3 major peaks at 28S, 18S and 4S on sucrose gradient centrifugation. The ratio of the amounts of 18S and 28S RNA increased significantly with age in the brain and liver. The polyacrylamide gel electrophoretic patterns of extracts of the 3 tissues under both native and denaturing conditions were nearly identical regardless of the age of the animals. Since most of the minor components separated on gels were probably in vivo degradation products of rRNA, the extent of apparent and hidden breaks in rRNA apparently did not change during aging.