Purification of the membrane-spanning tryptic peptides of the alpha polypeptide from sodium and potassium ion activated adenosinetriphosphatase labeled with 1-tritiospiro[adamantane-4,3'-diazirine].
Purification of the membrane-spanning tryptic peptides of the alpha polypeptide from sodium and potassium ion activated adenosinetriphosphatase labeled with 1-tritiospiro[adamantane-4,3'-diazirine].
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从用 1-tritiospiro[adamantane-4,3-diazirine] 标记的钠离子和钾离子激活的腺苷三磷酸酶中纯化 α 多肽的跨膜胰蛋白酶肽。
DOI：
10.1021/bi00300a015
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Nicholas,RA
中科院分区：
文献类型：
--
作者：
Nicholas,RA
Robert A.尼古拉斯摘要：从钠、钾离子激活的腺苷三磷酸酶[（Na ~++ K ~+）-ATP酶]的多肽中纯化了五个长的跨膜胰蛋白酶肽。(Na从犬肾中分离的（++ K+）-ATP酶在高浓度1-tritiospiro [金刚烷-4，3 '-diazirine]存在下暴露于紫外光，1-tritiospiro [金刚烷-4，3'-diazirine]是一种分配到膜双层中的卡宾前体。分离用1.2mol [3 H]金刚烷亚基（mol多肽）1修饰的α多肽并用胰蛋白酶消化。用胰蛋白酶消化可确保跨膜序列在消化过程中保持完整，因为赖氨酸和精氨酸具有极强的亲水性，很少出现在膜蛋白的膜包埋区域。这种消化产生了放射性胰蛋白酶
Robert A. Nicholas abstract: Five long, membrane-spanning tryptic peptides from the a polypeptide of sodium and potassium ion activated adenosinetriphosphatase [(Na++ K+)-ATPase] have been purified.(Na++ K+)-ATPase, isolated from canine kidney, was exposed to ultraviolet light in the presence of a high concentration of 1-tritiospiro [adamantane-4, 3'-diazirine], a carbene precursor that partitions into the bilayer of the membrane. The a polypeptide, modified with 1.2 mol of [3H] adamantylidene (mol of polypeptide)" 1, was isolated and digested with trypsin. Digestion with trypsin ensures that membrane-spanning sequences remain intact during the di-gestion, since lysine and arginine, being extremely hydrophilic, rarely appear in the membrane-embedded regions of mem-brane proteins. This digestion produced radioactive tryptic