EFFECTS OF COLCEMID INHIBITION AND REVERSAL ON FINE STRUCTURE OF MITOTIC APPARATUS OF CHINESE HAMSTER CELLS IN VITRO
EFFECTS OF COLCEMID INHIBITION AND REVERSAL ON FINE STRUCTURE OF MITOTIC APPARATUS OF CHINESE HAMSTER CELLS IN VITRO
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DOI：
10.1016/s0022-5320(67)80057-1
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发表时间：
1967-01-01
期刊：
影响因子：
--
通讯作者：
HSU, TC
中科院分区：
文献类型：
--
作者：
BRINKLEY, BR;STUBBLEFIELD, E;HSU, TC
用足以阻止有丝分裂的浓度（0.06 μ g/ml）的秋水仙胺处理中国仓鼠细胞，进行电子显微镜研究，结果表明，这种生物碱不能完全阻止有丝分裂器某些元素的形成。一个异常的机构形成了，其组织似乎是由中心分子的立场决定的。后一部分位于细胞中心附近，其余结构（微管、染色体和其他细胞器）呈放射状分布在它们周围。当秋水仙胺从培养基中取出时，整个细胞群在30[长破折号]45分钟内同步完成有丝分裂。只有在中心粒迁移到细胞的相对两端后，才形成完整的有丝分裂器。本研究描述了秋水仙胺抑制过程中细胞的超微结构和逆转过程中的各个阶段。在有丝分裂的初始阶段，正常的细胞分裂和遗传物质均匀分布到2个子细胞中似乎在很大程度上取决于中心粒的行为。秋水仙胺通过阻止中心粒运动所必需的有丝分裂器的某些元件（可能是连续纺锤体细丝的组装）的形成来抑制有丝分裂。
Electron microscope studies of Chinese hamster cells treated with Colcemid at a concentration sufficient to prevent mitosis (0.06 ug/nil) demonstrated that this alkaloid does not completely prevent the formation of certain elements of the mitotic apparatus. An aberrant apparatus formed whose organization appeared to be dictated by the position of the centrioles. The latter components assumed a position near the cell center, and the remaining structures (microtubules, chromosomes, and other organelles) became radially distributed around them. When Colcemid was removed from the culture medium, the entire cell population completed mitosis synchronously within 30[long dash]45 min. A complete mitotic apparatus formed only after the centrioles had migrated to opposite ends of the cells. The present study describes the ultrastructure of cells during Colcemid inhibition and at various stages during reversal. Normal cell division and the equidistribution of genetic material into 2 daughter cells appeared to be greatly dependent upon centriole behavior during the initial stages of mitosis. Colcemid inhibits mitosis by preventing the formation of certain elements of the mitotic apparatus (perhaps the assembly of continuous spindle filaments) necessary for centriole movement.