Metal exposures to native populations of the caddisfly Hydropsyche (Trichoptera: Hydropsychidae) determined from cytosolic and whole body metal concentrations
Metal exposures to native populations of the caddisfly Hydropsyche (Trichoptera: Hydropsychidae) determined from cytosolic and whole body metal concentrations
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DOI：
10.1023/a:1003583117293
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发表时间：
1998-01-01
期刊：
影响因子：
2.6
通讯作者：
Luoma, SN
中科院分区：
文献类型：
--
作者：
Cain, DJ;Luoma, SN
测定了水蚤体内细胞质(胞浆)和全身可溶部分的金属含量。(毛翅目)比较了连续四年(1992-1995年)沿克拉克福克河380公里污染梯度和一条受污染的支流(弗林特溪)采集的样本中细胞质和全身的金属积累情况。来自受污染地点的样本与未受污染的支流(布莱克福特河)进行了比较。胞液金属浓度和胞液蛋白(作为蛋白质代谢的一般生物标志物)之间的关系也在1994年和1995年进行了研究。相对于全身浓度，细胞内金属浓度因金属和年份而异。Cd、Cd镉、铜在胞质中的回收率为12%~%，铅为2%~38%。全身锌含量也与污染水平一致，但胞质锌浓度仅在全身锌浓度最高时升高。结果表明，锌在胞质中的回收率为16%~63%，且与全身锌浓度呈负相关。胞质金属的比例在不同年份之间差异很大，因此，胞质中金属浓度的年际差异大于全身。结果表明，河流中的水力压汞长期暴露于生物可利用的金属中。从全身浓度来看，这种暴露的一些特征并不明显。一般而言，蛋白质水平与胞液金属浓度并不对应。各种环境因素可以与金属暴露相互作用，在蛋白质代谢中产生复杂的反应。有必要进行系统研究，以区分多种环境应激源对生活在受污染生态系统中的生物体的影响。
Metal concentrations of the soluble fraction of the cytoplasm (cytosol) and the whole body were determined in the caddisfly Hydropsyche spp. (Trichoptera). Metal accumulation in the cytosol and the whole body were compared in samples collected along 380 kms of a contamination gradient in the Clark Fork river in four consecutive years (1992-1995), and from a contaminated tributary (Flint Creek). Samples from the contaminated sites were compared to an uncontaminated tributary (Blackfoot River). Relations between cytosolic metal concentration and cytosolic protein (used as a general biomarker of protein metabolism) also were examined in 1994 and 1995. Relative to whole body concentrations, cytosolic metal concentrations varied among metals and years. Spatial patterns in whole body and cytosolic Cd, Cu and Pb concentrations were qualitatively similar each year, and these concentrations generally corresponded to contamination levels measured in bed sediments. The proportions of metals recovered in the cytosol of ranged from 12 to 64% for Cd and Cu and from 2 to 38% for Pb. Zinc in the whole body also was consistent with contamination levels, but cytosolic Zn concentrations increased only at the highest whole body Zn concentrations. As a result, the proportion of Zn recovered in the cytosol ranged from 16 to 63% and tended to be inversely related to whole body Zn concentrations. The proportions of cytosolic metals varied significantly among years and, as a result, interannual differences in metal concentrations were greater in the cytosol than in the whole body. The results demonstrated that Hydropsyche in the river were chronically exposed to biologically available metals. Some features of this exposure were not evident from whole body concentrations. In general, protein levels did not correspond to cytosolic metal concentrations. A variety of environmental factors could interact with metal exposures to produce complex responses in protein metabolism. Systematic study will be necessary to differentiate the effects of multiple environmental stressors on organisms living in contaminated ecosystems.