STUDY OF YEAST ALPHA-GALACTOSIDASE WITH NAPHTHYL ALPHA-D-GALACTOPYRANOSIDES AS CHROMOGENIC SUBSTRATES
STUDY OF YEAST ALPHA-GALACTOSIDASE WITH NAPHTHYL ALPHA-D-GALACTOPYRANOSIDES AS CHROMOGENIC SUBSTRATES
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DOI：
10.1016/0003-2697(64)90239-8
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发表时间：
1964-01-01
影响因子：
2.9
通讯作者：
SU, HCF
中科院分区：
文献类型：
--
作者：
TSOU, KC;SU, HCF
从动力学角度研究了α-半乳糖酶的作用机理。比较它们的酶解率显示6-bromo-2-naphthyl>；1-naphthyl->；2-naph-thyl-[alpha]-D-galactopyranosides.这些数据表明，[α]-D-半乳糖苷中苷元基团的影响是空间加速的影响。得到了3-萘基[α]-D-半乳糖苷的Km和Vmax。醋酸汞对[α]-半乳糖苷酶有抑制作用，而半乳糖、葡萄糖、果糖、乳糖、半乳糖醛酸、半胱氨酸、组氨酸、咪唑和苯胺则无抑制作用。某些金属氰化物被发现是活化剂。
The mechanism of a-galactosi-dase action was studied kinetically. A comparison of the rate of their enzymic hydrolysis shows 6-bromo-2-naphthyl>1-naphthyl->2-naph-thyl-[alpha]-D-galactopyranosides. Such data suggest that the influence of the aglycone group in the [alpha]-D-galactosides is that of a steric acceleration. Km and Vmax for the 3 naphthyl [alpha]-D-galactopyranosides were obtained. Mercuric acetate was found to be an inhibitor for [alpha]-galacto sidase whereas galactose, glucose, fructose, lactose, galacturonic acid, cystelne, histidine, imidazole, and aniline were found to be noninhibi-tors. Certain metal cyanides were found to be activators.