A microfabricated array of clamps for immobilizing and imaging C-elegans
A microfabricated array of clamps for immobilizing and imaging C-elegans
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DOI：
10.1039/b707861g
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
Whitesides, George M.
中科院分区：
文献类型：
--
作者：
Hulme, S. Elizabeth;Shevkoplyas, Sergey S.;Whitesides, George M.
本文描述了一种用于快速固定大量活C.用于以高通量方式进行形态学分析、显微外科手术和荧光成像。该装置由两个主要元件组成：（i）128个楔形微通道或夹子的阵列，其物理地夹住蠕虫，以及（ii）分配通道的分支网络，其将蠕虫递送到阵列。通过该装置的液体流动（由入口和出口之间的恒定压差驱动）自动将单个蠕虫分配到每个夹具中。这是可能的，超过100蠕虫在不到15分钟的固定过程是没有损害的蠕虫：删除后，从阵列的夹具，蠕虫生活的典型寿命和繁殖正常。同时监测大量固定化蠕虫的能力将使研究人员能够研究大量C。优雅的
This paper describes the fabrication of a microfluidic device for rapid immobilization of large numbers of live C. elegans for performing morphological analysis, microsurgery, and fluorescence imaging in a high-throughput manner. The device consists of two principal elements: (i) an array of 128 wedge-shaped microchannels, or clamps, which physically immobilize worms, and (ii) a branching network of distribution channels, which deliver worms to the array. The flow of liquid through the device (driven by a constant pressure difference between the inlet and the outlet) automatically distributes individual worms into each clamp. It was possible to immobilize more than 100 worms in less than 15 min. The immobilization process was not damaging to the worms: following removal from the array of clamps, worms lived typical lifespans and reproduced normally. The ability to monitor large numbers of immobilized worms easily and in parallel will enable researchers to investigate physiology and behavior in large populations of C. elegans.