Photodynamic properties of ZnTPPS(4), ClAlPcS(2) and ALA in human melanoma G361 cells.
Photodynamic properties of ZnTPPS(4), ClAlPcS(2) and ALA in human melanoma G361 cells.
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DOI：
10.1016/j.tiv.2009.08.015
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发表时间：
2010-02
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影响因子：
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通讯作者：
E. Krestýn;Hana Kolarova;R. Bajgar;K. Tománková
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作者：
E. Krestýn;Hana Kolarova;R. Bajgar;K. Tománková
光动力疗法（PDT）已被批准为治疗某些类型的癌症和非恶性疾病的适当且有效的治疗方法。我们测试了锌-5,10,15,20-四(4-磺基苯基)卟啉 (ZnTPPS4)、酞菁二磺酸氯铝 (ClAlPcS2) 和 5-氨基乙酰丙酸 (ALA) 致敏的 G361 人黑色素瘤细胞的光动力效应。特别是，我们检查了 PDT 效率取决于所施加的光剂量（0.8；1.7；3.3；6.6；13.2；26.4Jcm−2）。采用DNA凝胶电泳、甲基噻唑四唑溴化物（MTT）活力测试、钙黄绿素AM和碘化丙啶（PI）染色荧光显微镜以及罗丹明123线粒体膜电位测定来检测和评估细胞死亡过程。我们还测量了活性氧（ROS）产生的时间过程及其对连续照射的敏化细胞内敏化剂浓度的依赖性。总之，这些结果表明，尽管 ZnTPPS4-PDT 在 G361 细胞上诱导 ROS 产生显着增加，但 ClAlPcS2-PDT 的光毒性作用最为显着。另一方面，ALA-PDT 具有最小的光效应，并且在下述条件下在 G361 细胞中诱导可忽略不计的 ROS 形成。
Photodynamic therapy (PDT) has been approved as proper and effective kind of treatment for certain types of cancer and non-malignant diseases. We tested photodynamic effects on G361 human melanoma cells sensitized by zinc-5,10,15,20-tetrakis(4-sulphonatophenyl) porphyrine (ZnTPPS4), chloraluminium phtalocyanine disulfonate (ClAlPcS2) and 5-aminolevulinic acid (ALA). In particular, we examined the PDT efficiency depending on applied light dose (0.8; 1.7; 3.3; 6.6; 13.2; 26.4Jcm−2). The DNA gel electrophoresis, methylthiazol tetrazolium bromide (MTT) viability test, fluorescent microscopy using calcein AM and propidium iodide (PI) staining, and rhodamine 123 mitochondrial membrane potential assay were performed to detect and evaluate the cell death process. We also measured the time course of reactive oxygen species (ROS) production and its dependence on sensitizer concentration within continuously irradiated sensitized cells. In conclusion, these results demonstrate most significant phototoxic effect of ClAlPcS2–PDT in spite of significantly higher ROS production induced by ZnTPPS4–PDT on G361 cells. On the other hand, ALA–PDT has a minimal photoeffect and induces negligible ROS formation in G361 cells at the conditions described below.