Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences.
Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences.
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DOI：
10.1084/jem.172.5.1377
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发表时间：
1990-11-01
期刊：
影响因子：
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通讯作者：
Feeney AJ
中科院分区：
文献类型：
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作者：
Feeney AJ
尽管表达γ / δ受体的胎儿T细胞缺乏N区，但T和B淋巴细胞受体的多样性大部分来自受体基因元件连接处非模板化N区的添加。我已经测序了免疫球蛋白H链可变区域的PCR扩增DNA和cDNA从胎儿和新生小鼠的肝脏和脾脏细胞。这些序列显示缺失N个区域。只有1/87的DNA序列和17/146的RNA序列含有N区，与成人Ig序列形成鲜明对比。这些数据表明，在B细胞和T细胞中，N区插入是一个受发育调节的过程，并表明新生B细胞中的受体多样性受到N区缺失和Vh基因使用的限制。
Much of T and B lymphocyte receptor diversity derives from the addition of nontemplated N regions at the junctions of receptor gene elements, although fetal T cells expressing gamma/delta receptors lack N regions. I have sequenced immunoglobulin H chain variable regions of PCR- amplified DNA and cDNA from fetal and newborn mouse liver and spleen cells. These sequences showed an absence of N regions. Only 1/87 DNA sequences and 17/146 RNA sequences contained N regions, in striking contrast to adult Ig sequences. These data show that N region insertion is a developmentally regulated process in B cells as well as in T cells, and demonstrate that receptor diversity in neonatal B cells is limited by the absence of N regions as well as by biased usage of Vh genes.