DIFFERENTIAL EFFECT OF CHRONIC NICOTINE ADMINISTRATION ON BRAIN CYTOCHROME-P4501A1/2 AND CYTOCHROME-P4502E1
DIFFERENTIAL EFFECT OF CHRONIC NICOTINE ADMINISTRATION ON BRAIN CYTOCHROME-P4501A1/2 AND CYTOCHROME-P4502E1
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DOI：
10.1006/bbrc.1993.1821
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发表时间：
1993-07-15
影响因子：
3.1
通讯作者：
WECKER, L
中科院分区：
文献类型：
--
作者：
ANANDATHEERTHAVARADA, HK;WILLIAMS, JF;WECKER, L
测定了慢性尼古丁对小鼠脑区和肝脏微体部分P4501A1/2和P4502E1酶活性和免疫活性的影响。慢性尼古丁可增加大鼠海马区和脑干区的7-乙氧基间苯二酚O-脱乙基酶活性和P4501A1/2免疫反应性，降低大脑皮层、丘脑和纹状体的P4501A1/2免疫反应性，但对其他脑区的参数无影响。相比之下，尼古丁注射大鼠的N-亚硝基二甲胺-N-脱甲基酶活性在所有被检查的脑区都增加了，并且这种增加与P4502E1免疫反应性的增加有关。慢性尼古丁不改变肝微粒体中这些异构体的酶活性或免疫反应性。
The effects of chronic nicotine administration on the enzymatic activities and immunoreactivities of P4501A1/2 and P4502E1 were determined in microsomal fractions from brain regions and liver. Chronic nicotine increased 7-ethoxyresorufin O-deethylase activity and P4501A1/2 immunoreactivity in hippocampus and brain stem, decreased values in cerebral cortex, thalamus, and striatum, and did not affect parameters in other brain regions. In contrast, N-nitrosodimethylamine N-demethylase activity from nicotine-injected rats increased in all brain regions examined, and this increase was associated with increased P4502E1 immunoreactivity. Chronic nicotine did not alter either enzyme activities or immunoreactivities of these isoforms in hepatic microsomes.