Characteristic effects of methylglyoxal and its degraded product formate on viability of human histiocytes: a possible detoxification pathway of methylglyoxal.
Characteristic effects of methylglyoxal and its degraded product formate on viability of human histiocytes: a possible detoxification pathway of methylglyoxal.
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DOI：
10.1016/j.bbrc.2011.03.041
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发表时间：
2011-04
影响因子：
3.1
通讯作者：
K. Koizumi;M. Nakayama;Wanjun Zhu;S. Ito
中科院分区：
文献类型：
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作者：
K. Koizumi;M. Nakayama;Wanjun Zhu;S. Ito
甲基乙二醛（MGO）是一种毒性和高度反应性的α-乙醛，在各种疾病的状态下升高，这些疾病是氧化应激增强的基础。此外，已报道MGO产生另一种醛，甲酸（FA）。因此，研究FA的生物学特性具有重要意义。本研究使用U937人组织细胞系检查MGO和FA对细胞活力的影响。FA显示在细胞活力降低的MGO浓度下细胞活力呈剂量依赖性增加。FA增加细胞活力的机制涉及叶酸途径中内源性过氧化氢（H2 O2）和四氢叶酸的存在，而MGO降低细胞活力的机制涉及与H2 O2和氧化损伤的相互作用。这些研究结果表明，FA生产的MGO降解可能发挥作用，在衰减的氧化性细胞损伤所造成的MGO。我们推测FA生成途径构成了MGO的解毒系统。
Methylglyoxal (MGO) is a toxic and highly reactive alpha-oxoaldehyde, elevated in the states of various diseases underlying enhanced oxidative stress. Furthermore, MGO has been reported to generate another aldehyde, formic acid (FA). In this sense, investigating the biological property of FA is crucially important. The present study examined the effects of MGO and FA on cell viability using the U937 human histiocytic cell line. FA showed a dose-dependent increase in cell viability at the concentrations of MGO in which cell viability decreased. The mechanism of the increase by FA involved the presence of endogenous hydrogen peroxide (H2O2) and tetrahydrofolate in the folate pathway, whereas that of the decrease in cell viability by MGO involved interaction with H2O2and oxidative damage. These findings suggest that FA production by MGO degradation may play a role in attenuation of oxidative cellular injury caused by MGO. We hypothesize that FA generation pathway constitutes a detoxification system for MGO.