INVITRO AND INVIVO RADIOSENSITIVITY OF HUMAN TUMOR-CELLS OBTAINED FROM A PANCREATIC CARCINOMA XENOGRAFT
INVITRO AND INVIVO RADIOSENSITIVITY OF HUMAN TUMOR-CELLS OBTAINED FROM A PANCREATIC CARCINOMA XENOGRAFT
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DOI：
10.1038/263771a0
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发表时间：
1976-01-01
期刊：
影响因子：
64.8
通讯作者：
STEEL, GG
中科院分区：
文献类型：
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作者：
COURTENAY, VD;SMITH, IE;STEEL, GG
人类肿瘤对放射治疗表现出广泛的临床反应，但这种反应在多大程度上反映了细胞固有的放射敏感性、肿瘤细胞缺氧的程度或宿主对肿瘤的反应尚不清楚。对于实验动物的肿瘤，克隆形成细胞的各种检测方法都是可用的。这些结果表明，需氧细胞的辐射敏感性范围相当窄，1，2在可触及的肿瘤内克隆细胞的低氧比例通常在10-35%。到目前为止，缺乏令人满意的克隆细胞检测方法，阻碍了对体内治疗的人类实体肿瘤进行类似的研究。我们已经建立了两种方法来检测人转移性胰腺癌在免疫抑制的CBA/lac小鼠中作为异种移植瘤的克隆性细胞。这种异种移植是Pickardet等人建立的一系列移植之一。
HUMAN tumours show a wide range of clinical response to radiotherapy, but the extent to which this reflects differences in intrinsic cellular radiosensitivity, extent of tumour cell hypoxia, or host reaction against the tumour is unknown. For tumours in laboratory animals, various assays for clonogenic cells are available. These have shown that the range of radiosensitivity of aerobic cells is quite narrow1,2and that the hypoxic fraction of clonogenic cells within tumours of palpable dimensions is often in the range 10–35%. Until now the lack of satisfactory assay for clonogenic cells has prevented similar studies on human solid tumours treatedin vivo. We have developed two assays for clonogenic cells from a human metastatic pancreatic carcinoma propagated as a xenograft in immune-suppressed CBA/lac mice. This xenograft was one of a series established by Pickardet al.3.