Cytotoxic electronegative LDL subfraction is present in human plasma
Cytotoxic electronegative LDL subfraction is present in human plasma
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DOI：
10.1161/01.atv.16.6.773
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发表时间：
1996-06-01
影响因子：
8.7
通讯作者：
Moatti, N
中科院分区：
文献类型：
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作者：
Demuth, K;Myara, I;Moatti, N
通过使用快速蛋白质液相色谱，我们从人血浆中分离出少量的电阴性低密度脂蛋白亚组分，命名为低密度脂蛋白(-)。经载脂蛋白(A)和载脂蛋白A-I免疫亲和层析后，低密度脂蛋白(-)占总低密度脂蛋白的6.7+/-0.9%(平均+/-SD；n=18)。与主要的低密度脂蛋白亚组分相比，低密度脂蛋白(-)含有类似数量的硫代巴比妥酸反应物质、共轭双烯和维生素E，并且具有相似的脂/蛋白比和平均密度。此外，低密度脂蛋白(-)的载脂蛋白B没有聚集，其低密度脂蛋白受体结合活性略有增加。这些结果与低密度脂蛋白(-)的非氧化性相一致。低密度脂蛋白(-)组唾液酸含量(38.1+/-5.2)明显高于对照组(28.9+/-3.3nmol/mg蛋白；n=7；P
By using fast protein liquid chromatography, we isolated from human plasma a minor electronegative LDL subfraction designated LDL(-). After immunoaffinity chromatography against apolipoprotein (apo)(a) and apo A-I, LDL(-) represented 6.7+/-0.9% (mean+/-SD; n=18) of to al LDL. Compared with the major LDL subfraction, designated LDL(+), LDL(-) contained similar amounts of thiobarbituric acid-reactive substances, conjugated dienes, and vitamin E and had a similar lipid/protein ratio and mean density. Moreover, the apo B of LDL(-) was not aggregated and its LDL receptor-binding activity was slightly increased. These results were consistent with the nonoxidized nature of LDL(-). LDL(-) showed increased contents of sialic acid (38.1+/-5.2 versus 28.9+/-3.3 nmol/mg protein; n=7; P