Age and rearing environment interact in the retention of early olfactory memories in honeybees
Age and rearing environment interact in the retention of early olfactory memories in honeybees
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DOI：
10.1007/s00359-008-0337-z
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发表时间：
2008-07-01
影响因子：
2.1
通讯作者：
Farina, Walter M.
中科院分区：
文献类型：
--
作者：
Arenas, Andres;Farina, Walter M.
由于群居昆虫在成年期间暴露的行为环境不断变化，因此它们是分析成年早期特定感官刺激对以后行为影响的理想模型。具体来说，我们的目标是了解早期对后期觅食行为的影响。为此，工蜂利用（1）长鼻延伸反应（PER）范式中的经典条件反射或（2）在不同饲养条件下提供香味糖溶液，在不同的觅食前年龄建立了嗅觉记忆。通过对采食年龄（17-25 天）工蜂进行单次 PER 测试来测试长期记忆 (LTM)，我们发现蜜蜂早期嗅觉记忆的保留与年龄相关，而不是与时间相关。与饲养环境条件（实验室笼子或蜂巢）无关，蜜蜂能够在羽化后 5 天保留食物与气味的关联，但在此之前很少见。在大多数实验中，我们观察到双峰反应模式：在 5-8 天和 13-16 天接触有香味的食物的蜜蜂比在 9-12 天接触的蜜蜂表现出更好的保留能力。对于在蜂巢中饲养的蜜蜂来说，这些差异消失了。讨论了根据适当感官刺激的时间和连续输入来检索 LTM。
Due to the changing behavioral contexts at which social insects are exposed during the adult lifespan, they are ideal models to analyze the effect of particular sensory stimuli during young adulthood on later behavior. Specifically, our goal is to understand early influences on later foraging behavior. For that, olfactory memories were established by worker honeybees to different pre-foraging ages using either (1) classical conditioning in the proboscis extension response (PER) paradigm or (2) the offering of scented-sugar solution under different rearing conditions. By testing long-term memories (LTM) through a single PER test in workers of foraging ages (17-25 days), we found that retention of the early olfactory memories in honey bees is age-dependent and not time-dependent. Independently of the environmental conditions in which they were reared (laboratory cages or hives), bees were able to retain food-odor association from 5 days after emergence, but rarely before. In most experiments we observed a bi-modal pattern of response: bees exposed to scented-food at 5-8 and 13-16 days showed better retention than those exposed at 9-12 days. These differences disappeared for bees reared in hives. Retrieval of LTMs depending on the timing and the continuous inputs of appropriate sensory stimuli are discussed.