Comparative mapping of the Oregon Wolfe Barley using doubled haploid lines derived from female and male gametes
Comparative mapping of the Oregon Wolfe Barley using doubled haploid lines derived from female and male gametes
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DOI：
10.1007/s00122-011-1540-9
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发表时间：
2011-05-01
影响因子：
5.4
通讯作者：
Hayes, P. M.
中科院分区：
文献类型：
--
作者：
Cistue, L.;Cuesta-Marcos, A.;Hayes, P. M.
俄勒冈州沃尔夫·大麦群体绘图是遗传学研究和教学的资源。先前的报告基于通过球根大麦 (H.b.) 方法开发的双单倍体 (DH) 系群体，该方法对雌配子进行取样。我们使用花药培养物 (A.C.) 从同一杂交中开发了新的 DH 系，该花药培养物对雄配子进行取样。使用相同的 1,328 个单核苷酸多态性标记在两个亚群中的每一个中生成连锁图谱。基于衍生自大孢子发生和小孢子发生产物的DH系的连锁图谱揭示了估计重组率方面的微小差异。基因座排序没有差异。 A.C. 来源的亚群中存在比 H.b. 来源的亚群更大的分离扭曲，但在显示最大扭曲的区域中，原因更可能是 ZEO1 株高基因座的等位基因变异，而不是 DH 生产方法。隔离扭曲和多效性的影响对数量性状基因座效应的估计的影响比在温室条件下测定的生殖适合性状的群体规模的影响更大。俄勒冈州沃尔夫大麦 (OWB) 人口和数据是社区资源。种子可从位于北美、欧洲和亚洲的三个配送中心获得。有关订购种子集的详细信息以及完整的基因型和表型数据文件，请访问 http://wheat.pw.usda.gov/ggpages/maps/OWB/。
The Oregon Wolfe Barley mapping population is a resource for genetics research and instruction. Prior reports are based on a population of doubled haploid (DH) lines developed by the Hordeum bulbosum (H.b.) method, which samples female gametes. We developed new DH lines from the same cross using anther culture (A.C.), which samples male gametes. Linkage maps were generated in each of the two subpopulations using the same 1,328 single nucleotide polymorphism markers. The linkage maps based on DH lines derived from the products of megasporogeneis and microsporogenesis revealed minor differences in terms of estimated recombination rates. There were no differences in locus ordering. There was greater segregation distortion in the A.C.-derived subpopulation than in the H.b.-derived subpopulation, but in the region showing the greatest distortion, the cause was more likely allelic variation at the ZEO1 plant height locus rather than to DH production method. The effects of segregation distortion and pleiotropy had greater impacts on estimates of quantitative trait locus effect than population size for reproductive fitness traits assayed under greenhouse conditions. The Oregon Wolfe Barley (OWB) population and data are community resources. Seed is available from three distribution centers located in North America, Europe, and Asia. Details on ordering seed sets, as well as complete genotype and phenotype data files, are available at http://wheat.pw.usda.gov/ggpages/maps/OWB/.