Mapping of QTL controlling NIR predicted hot water extract and grain nitrogen content in a spring barley cross using marker-regression
Mapping of QTL controlling NIR predicted hot water extract and grain nitrogen content in a spring barley cross using marker-regression
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DOI：
10.1111/j.1439-0523.1997.tb02168.x
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发表时间：
1997-05-01
期刊：
影响因子：
2
通讯作者：
Kearsey, MJ
中科院分区：
文献类型：
--
作者：
Bezant, JH;Laurie, DA;Kearsey, MJ
利用99个春大麦品种（Blenheim × Kym）杂交的双单倍体株系构建限制性片段长度多态性（RFLP）图谱，定位了控制热水浸出物和籽粒氮含量的QTL（近红外反射光谱分析预测）。八个QTL影响预测热水提取物的标记回归方法进行了鉴定。最大的影响被发现在染色体3HL，与denso矮化基因，这是目前在'布伦海姆'，并赋予较差的预测热水提取物的质量，和4HL。在1HS、1HL、2HS、2HL、5HL和6HS染色体上检测到其它QTL。通过方差分析的单个标记的分析检测到染色体1H上的额外影响。通过分子标记回归分析，在1HS、1HL、2HL、5HS、6H、7HS和7HL染色体上共检测到8个影响籽粒氮含量的QTL。也有证据表明在染色体5HL上存在额外的QTL。位于5HS和5HL上的籽粒氮含量QTL与位于小麦轮状病毒5A和5D染色体上的影响籽粒蛋白质含量的QTL位置相当。denso基因对籽粒含氮量无明显影响。
A restriction fragment length polymorphism (RFLP) map constructed from 99 doubled haploid lines of a cross between two spring barley varieties ('Blenheim' x 'Kym') was used to map QTL controlling hot water extract and grain nitrogen content (predicted by analysis with near-infrared reflectance spectroscopy). Eight QTL affecting predicted hot water extract were identified by a marker-regression approach. The largest effects were found on chromosomes 3HL, associated with the denso dwarfing gene which is present in 'Blenheim' and conferred poorer predicted hot water extract quality, and 4HL. Other QTL were detected on chromosomes 1HS, 1HL, 2HS, 2HL, 5HL and 6HS. Analysis of single markers by analysis of variance detected an additional effect on chromosome 1H. Eight QTL affecting predicted grain nitrogen content were identified by marker-regression, on chromosomes 1HS, 1HL, 2HL, 5HS, 6H, 7HS and 7HL. There was also evidence for an additional QTL on chromosome 5HL. The positions of the grain nitrogen content QTL on 5HS and 5HL are comparable to QTL on wheal chromosomes 5A and 5D that affect grain protein content. The denso gene had no detectable effect on grain nitrogen content.